School of Medicine, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
Research Center in Tropical Pathobiology, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
Sci Rep. 2023 Nov 3;13(1):18987. doi: 10.1038/s41598-023-46431-2.
Naturally occurring DNA gaps have been observed in eukaryotic DNA, including DNA in nondividing cells. These DNA gaps are found less frequently in chronologically aging yeast, chemically induced senescence cells, naturally aged rats, D-galactose-induced aging model rats, and older people. These gaps function to protect DNA from damage, so we named them youth-associated genomic stabilization DNA gaps (youth-DNA-gaps). Type 2 diabetes mellitus (type 2 DM) is characterized by an early aging phenotype. Here, we explored the correlation between youth-DNA-gaps and the severity of type 2 DM. Here, we investigated youth-DNA-gaps in white blood cells from normal controls, pre-DM, and type 2 DM patients. We found significantly decreased youth-DNA-gap numbers in the type 2 DM patients compared to normal controls (P = 0.0377, P = 0.0018 adjusted age). In the type 2 DM group, youth-DNA-gaps correlate directly with HbA1c levels. (r = - 0.3027, P = 0.0023). Decreased youth-DNA-gap numbers were observed in patients with type 2 DM and associated with increased HbA1c levels. Therefore, the decrease in youth-DNA-gaps is associated with the molecular pathogenesis of high blood glucose levels. Furthermore, youth-DNA-gap number is another marker that could be used to determine the severity of type 2 DM.
真核生物 DNA 中存在自然产生的 DNA 缺口，包括非分裂细胞中的 DNA。这些 DNA 缺口在chronologically aging yeast、chemically induced senescence cells、naturally aged rats、D-galactose-induced aging model rats 和老年人中较少出现。这些缺口的功能是保护 DNA 免受损伤，因此我们将其命名为与年轻相关的基因组稳定化 DNA 缺口（youth-DNA-gaps）。2 型糖尿病（type 2 DM）的特征是早期衰老表型。在这里，我们探讨了 youth-DNA-gaps 与 2 型 DM 严重程度之间的相关性。在这里，我们研究了来自正常对照、pre-DM 和 2 型 DM 患者的白细胞中的 youth-DNA-gaps。与正常对照组相比，2 型 DM 患者的 youth-DNA-gap 数量明显减少（P=0.0377，P=0.0018 调整年龄）。在 2 型 DM 组中，youth-DNA-gaps 与 HbA1c 水平直接相关（r=-0.3027，P=0.0023）。在 2 型 DM 患者中观察到 youth-DNA-gap 数量减少，与 HbA1c 水平升高相关。因此，youth-DNA-gap 数量的减少与高血糖水平的分子发病机制有关。此外，youth-DNA-gap 数量是另一个可用于确定 2 型 DM 严重程度的标志物。
