Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool , Liverpool, United Kingdom.
Medical Laboratory Technology Department, Northern Border University , Arar City, Saudi Arabia.
J Virol. 2023 Nov 30;97(11):e0042423. doi: 10.1128/jvi.00424-23. Epub 2023 Nov 6.
SARS-CoV-2 has caused a worldwide health and economic crisis. During the course of the pandemic, genetic changes occurred in the virus, which have resulted in new properties of the virus-particularly around gains in transmission and the ability to partially evade either natural or vaccine-acquired immunity. Some of these viruses have been labeled Variants of Concern (VoCs). At the root of all VoCs are two mutations, one in the viral spike protein that has been very well characterized and the other in the virus polymerase (NSP12). This is the viral protein responsible for replicating the genome. We show that NSP12 associates with host cell proteins that act as a scaffold to facilitate the function of this protein. Furthermore, we found that different variants of NSP12 interact with host cell proteins in subtle and different ways, which affect function.
SARS-CoV-2 引发了全球性的健康和经济危机。在大流行过程中，病毒发生了遗传变化，导致病毒产生了新的特性——特别是在传播能力的提高和部分逃避自然或疫苗获得的免疫能力方面。其中一些病毒已被标记为关切变种（VOCs）。所有 VOC 的根源都是两个突变，一个在病毒的刺突蛋白上，这个突变已经得到了很好的描述，另一个在病毒聚合酶（NSP12）上。这是负责复制基因组的病毒蛋白。我们表明，NSP12 与宿主细胞蛋白结合，作为一个支架来促进该蛋白的功能。此外，我们发现 NSP12 的不同变体以微妙和不同的方式与宿主细胞蛋白相互作用，从而影响其功能。
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