Sztandera-Tymoczek Monika, Wdowiak-Wróbel Sylwia, Świderska Urszula, Palusińska-Szysz Marta, Szuster-Ciesielska Agnieszka
Department of Virology and Immunology, Institute of Biological Sciences, Maria Curie-Skłodowska University, Lublin, Poland.
Department of Genetics and Microbiology, Institute of Biological Sciences, Maria Curie-Skłodowska University, Lublin, Poland.
J Inflamm Res. 2023 Nov 1;16:5039-5060. doi: 10.2147/JIR.S425383. eCollection 2023.
Allergic diseases have reached epidemic proportions globally, affecting nearly 30% of the world's population. One of the most prominent sources of allergens is fungi, causing up to 6% of respiratory diseases in the general population. However, the cause of respiratory allergies is not always identifiable. Therefore, we studied the ability of two representatives of common powdery mildew (Erysiphales), and , to induce a proinflammatory response in in vitro models of the upper and lower respiratory tract.
Two cell lines, BEAS-2B and A549, were used to mimic upper and lower respiratory epithelial cells. The toxicity of fungal extracts was assessed with MTT and flow cytometry assay. The production of reactive oxygen species in the cells was measured with flow cytometry. ELISA tests were used to determine the production of proinflammatory cytokines. The presence of the cell integrity marker was assessed with the immunofluorescence method.
In both cell lines, the extract of and microfungi induced marked production of proinflammatory IL-1β, TNF-α, and GM-CSF cytokines involved in developing allergic reactions. The higher levels of these cytokines with higher reactive oxygen species synthesis positively correlated with the disruption of epithelial cell junctions.
We conclude that and microfungi have strong proinflammatory and proallergenic potential, but this finding needs in vivo confirmation.
过敏性疾病在全球已达到流行程度，影响着近30%的世界人口。变应原最主要的来源之一是真菌，在普通人群中导致高达6%的呼吸道疾病。然而，呼吸道过敏的病因并非总是可识别的。因此，我们研究了常见白粉菌（白粉菌目）的两个代表种，即[具体种名1]和[具体种名2]，在上、下呼吸道体外模型中诱导促炎反应的能力。
使用两种细胞系BEAS - 2B和A549来模拟上、下呼吸道上皮细胞。用MTT和流式细胞术检测法评估真菌提取物的毒性。用流式细胞术测量细胞中活性氧的产生。用ELISA试验测定促炎细胞因子的产生。用免疫荧光法评估细胞完整性标志物的存在情况。
在两种细胞系中，[具体种名1]和[具体种名2]微真菌的提取物均诱导了参与过敏反应发展的促炎细胞因子IL - 1β、TNF - α和GM - CSF的显著产生。这些细胞因子水平较高且活性氧合成水平较高与上皮细胞连接的破坏呈正相关。
我们得出结论，[具体种名1]和[具体种名2]微真菌具有很强的促炎和促过敏潜力，但这一发现需要体内实验的证实。
