[聚尿苷酸与大肠杆菌核糖体30S亚基相互作用的定量研究]
[Quantitative studies of interaction of polyuridylic acid with 30S subunits of ribosomes of Escherichia coli].
作者信息
Kirillov S V, Katunin V I, Makhno V I, Semenkov Iu P
出版信息
Mol Biol (Mosk). 1979 May-Jun;13(3):690-7.
Polyuridilic acid of average molecular weight 18 000 binds to the 30S subunits with stoichiometry 1 : 1 but two kinds of 30S.poly(U) complexes with different stability are formed. The main reason for such heterogeneity was found to be due to the presence or absence of ribosomal protein Sl in 30S subunits. In its presence the association constant of 30S.poly(U) complex is equal 2.7.10(8) M-1, and in the opposite case it is much less 1.5.10(6) M-1. In the same conditions (20 mM MgCl2, 200 mM HN4Cl, 0 degrees) the association constant of binary complex Sl.poly(U) is equal 5.10(7) M-1.
平均分子量为18000的聚尿苷酸以化学计量比1:1与30S亚基结合，但形成了两种稳定性不同的30S·聚（U）复合物。发现这种异质性的主要原因是30S亚基中存在或不存在核糖体蛋白S1。在其存在的情况下，30S·聚（U）复合物的缔合常数等于2.7×10⁸ M⁻¹，而在相反的情况下则小得多，为1.5×10⁶ M⁻¹。在相同条件下（20 mM MgCl₂、200 mM NH₄Cl、0℃），二元复合物S1·聚（U）的缔合常数等于5×10⁷ M⁻¹。
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