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韩国阳光玫瑰葡萄上与蝇粪病相关的病原菌的系统发育和形态学特征

Phylogenetic and Morphological Characterization of Associated with Flyspeck on Shine Muscat Grapes in South Korea.

作者信息

Hassan Oliul, Lim Yang-Sook, Chang Taehyun

机构信息

Department of Ecology & Environmental System, College of Ecology & Environmental Sciences, Kyungpook National University, Sangju, Korea.

Gyeongsangbuk-do Agricultural Research & Extension Services, Daegu, Korea.

出版信息

Mycobiology. 2021 Mar 26;49(2):183-187. doi: 10.1080/12298093.2021.1894046. eCollection 2021.

DOI:10.1080/12298093.2021.1894046
PMID:37970185
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10635110/
Abstract

The Shine Muscat is a table grape, popular in South Korea for its unique mango-flavor taste. Flyspeck is a disease that is characterized by small, black, and circular specks on the grape cuticle was first observed in several commercial orchards in Sangju, South Korea, in August 2019. Here we identified the causal agent of flyspeck based on an advanced diagnosis approach, comprised of both morphological and molecular analyses. Morphological characteristics of the cultures isolated from grape flyspeck were identical to the fungus . The concatenated sequences of ITS, , and were used for molecular phylogenetic analysis, BLAST searches along with Bayesian inference-based phylogeny, confirmed that the causal agent of grape flyspeck is . . The cultured fungal isolates also produced flyspeck symptoms on healthy fruits in pathogenicity tests. To the best of my knowledge, this is the first documented evidence of any sp. producing flyspeck symptoms on any plant.

摘要

阳光玫瑰葡萄是一种鲜食葡萄,因其独特的芒果味在韩国很受欢迎。蝇粪病是一种以葡萄表皮出现小黑点和圆形斑点为特征的病害,2019年8月在韩国尚州的几个商业果园首次被发现。在这里,我们基于一种先进的诊断方法,包括形态学和分子分析,鉴定了蝇粪病的病原体。从葡萄蝇粪病分离出的培养物的形态特征与该真菌相同。ITS、 和 的串联序列用于分子系统发育分析,通过BLAST搜索以及基于贝叶斯推断的系统发育分析,证实葡萄蝇粪病的病原体是 。 。在致病性测试中,培养的真菌分离物也在健康果实上产生了蝇粪病症状。据我所知,这是任何 种在任何植物上产生蝇粪病症状的首个文献记载证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/278c/10635110/1ad3f0859627/TMYB_A_1894046_F0003_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/278c/10635110/e01bdc1eda36/TMYB_A_1894046_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/278c/10635110/324994cc1c57/TMYB_A_1894046_F0002_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/278c/10635110/1ad3f0859627/TMYB_A_1894046_F0003_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/278c/10635110/e01bdc1eda36/TMYB_A_1894046_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/278c/10635110/324994cc1c57/TMYB_A_1894046_F0002_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/278c/10635110/1ad3f0859627/TMYB_A_1894046_F0003_B.jpg