双极射频引起皮肤收缩的实验研究。
Experimental Study of Skin Contraction Induced by Bipolar Radiofrequency.
出版信息
Altern Ther Health Med. 2024 Aug;30(8):176-181.
BACKGROUND
Facial skin relaxation has become an important part in solving the problem of facial rejuvenation. Minimally invasive or noninvasive skin-tightening procedures have become a trend for facial rejuvenation. Bipolar radiofrequency (RF) is a new option for treating skin relaxation and is more effective than noninvasive surgery without surgical incision.
OBJECTIVE
To explore the effect of different bipolar RF powers on the area of the original box, changes of skin and subcutaneous tissue thickness and numbers of fibroblasts in rabbits.
DESIGN
The research team performed an animal study.
SETTING
This study took place in Affiliated Hospital of Nanjing University of Chinese Medicine.
PARTICIPANTS
Eighteen common-grade adult New Zealand rabbits (female, 2.5-3.0 kg).
METHODS
Bipolar radiofrequency therapy was given to a girl rabbit on the left side of the treatment area. Standard HE and Masson staining were performed to assess the pathological changes, area of the original box and the number of fibroblasts in skin and subcutaneous tissues.
OUTCOME MEASURES
(1) The area of the original box, changes of skin and subcutaneous tissue thickness, and numbers of fibroblasts under different bipolar RF temperatures or under different bipolar RF powers immediately after surgery, 1 month after surgery and 3 months after surgery were observed. (2) Standard HE and Masson staining results.
RESULTS
Under the condition of certain instrument power, at 36de 38d and 40nd the area of the original box shrank to different degrees immediately after surgery (16.54±0.37, 17.78±0.03, 17.19±0.01), 1 month after surgery (16.59±0.31, 17.82±0.01, 18.34±0.30) and 3 months after surgery (16.89±0.12, 18.16±0.14, 19.23±0.32) compared with that before surgery (P < .05). Under specific temperature conditions, at 16 W, 18 W, 20 W, and 22 W, the area of the original box shrank to different degrees immediately after surgery (16.40±0.49, 15.55±0.57, 17.54±0.12, 16.19±0.27), 1 month after surgery (16.88±0.12, 17.46±0.02, 18.05±0.35, 19.41±0.08) and 3 months after surgery (19.09±1.01, 18.30±0.69, 20.00±0.29, 21.20±0.90) compared with that before surgery (P < .05). When the power was fixed, the thickness of skin and subcutaneous tissue decreased immediately after surgery (6.7, 6.8, 7), 1 month after surgery (6, 6.1, 6.3) and 3 months after surgery (6.4, 6.5, 6.2) at different temperatures (P < .05). When the temperature was fixed, the thickness of skin and subcutaneous tissue decreased immediately after surgery (6.1, 6.08, 6.03), 1 month after surgery (6.2, 6.15, 6.13), and 3 months after surgery (6.2, 6.23, 6.03) under different powers (P < .05). Under the condition of certain instrument power, at 36de 38d and 40n, the number of fibroblasts increased to different degrees immediately after surgery (26.54±2.37, 30.78±3.03, 37.19±4.01), 1 month after surgery (28.59±2.31, 34.82±3.01, 40.34±4.30), and 3 months after surgery (30.89±0.12, 38.16±0.14, 42.23±0.32) compared with that before surgery, and all were statistically significant (P < .05). Under specific temperature conditions, at 16 W, 18 W, 20 W, and 22 W, the number of fibroblasts increased to different degrees immediately after surgery (28.29±2.49, 30.97±3.57, 38.74±3.12, 45.68±4.27), 1 month after surgery (30.88±3.12, 32.46±4.02, 41.05±0.35, 50.41±0.08), and 3 months after surgery (29.99±2.01, 33.30±2.69, 39.00±3.29, 23.20±2.90) compared with that before surgery, and all were statistically significant (P < .05).
CONCLUSIONS
Our study clarifies that bipolar RF can decrease the skin and subcutaneous tissue thickness and increase the numbers of fibroblasts at the temperature of 36°C, 38°C, and 40°C and frequency of 16-22 W, which has a therapeutical effect on skin contraction. Our study might effectively improve the skin slack of patients, and the postoperative maintenance rate is high, and will not cause obvious complications. This study may provide a theoretical direction for clinicians to tighten the skin of patients using bipolar RF.
背景
面部皮肤松弛已成为解决面部年轻化问题的重要部分。微创或非侵入性的皮肤紧致程序已成为面部年轻化的一种趋势。双极射频（RF）是一种治疗皮肤松弛的新选择，比非侵入性手术更有效，且无需手术切口。
目的
探讨不同双极 RF 功率对兔皮肤和皮下组织厚度及成纤维细胞数量的影响。
设计
该研究为动物研究团队进行的研究。
地点
南京中医药大学附属医院。
参与者
18 只普通级成年新西兰兔（雌性，2.5-3.0 kg）。
方法
在治疗区域的左侧对一只雌性兔子进行双极射频治疗。采用标准 HE 和 Masson 染色评估病理变化、原始盒的面积以及皮肤和皮下组织中成纤维细胞的数量。
观察指标
（1）不同双极 RF 温度或不同双极 RF 功率下，术后即刻、术后 1 个月和术后 3 个月的原始盒面积、皮肤和皮下组织厚度变化以及成纤维细胞数量。（2）标准 HE 和 Masson 染色结果。
结果
在一定仪器功率的条件下，36°C、38°C 和 40°C 时，术后即刻原始盒面积分别缩小至 16.54±0.37、17.78±0.03 和 17.19±0.01（P<.05），1 个月后分别缩小至 16.59±0.31、17.82±0.01 和 18.34±0.30（P<.05），3 个月后分别缩小至 16.89±0.12、18.16±0.14 和 19.23±0.32（P<.05）。在特定温度条件下，16 W、18 W、20 W 和 22 W 时，术后即刻原始盒面积分别缩小至 16.40±0.49、15.55±0.57、17.54±0.12 和 16.19±0.27（P<.05），1 个月后分别缩小至 16.88±0.12、17.46±0.02、18.05±0.35 和 19.41±0.08（P<.05），3 个月后分别缩小至 19.09±1.01、18.30±0.69、20.00±0.29 和 21.20±0.90（P<.05）。当功率固定时，术后即刻皮肤和皮下组织厚度分别变薄至 6.7、6.8 和 7（P<.05），1 个月后分别变薄至 6.0、6.1 和 6.3（P<.05），3 个月后分别变薄至 6.4、6.5 和 6.2（P<.05）。当温度固定时，术后即刻皮肤和皮下组织厚度分别变薄至 6.1、6.08 和 6.03（P<.05），1 个月后分别变薄至 6.2、6.15 和 6.13（P<.05），3 个月后分别变薄至 6.2、6.23 和 6.03（P<.05）。在一定仪器功率的条件下，36°C、38°C 和 40°C 时，术后即刻成纤维细胞数量分别增加至 26.54±2.37、30.78±3.03 和 37.19±4.01（P<.05），1 个月后分别增加至 28.59±2.31、34.82±3.01 和 40.34±4.30（P<.05），3 个月后分别增加至 30.89±0.12、38.16±0.14 和 42.23±0.32（P<.05）。在特定温度条件下，16 W、18 W、20 W 和 22 W 时，术后即刻成纤维细胞数量分别增加至 28.29±2.49、30.97±3.57、38.74±3.12 和 45.68±4.27（P<.05），1 个月后分别增加至 30.88±3.12、32.46±4.02、41.05±0.35 和 50.41±0.08（P<.05），3 个月后分别增加至 29.99±2.01、33.30±2.69、39.00±3.29 和 23.20±2.90（P<.05）。
结论
本研究阐明，双极 RF 在 36°C、38°C 和 40°C 温度和 16-22 W 频率下可减少皮肤和皮下组织厚度，增加成纤维细胞数量，对皮肤收缩有治疗作用。本研究可能有效改善患者的皮肤松弛，且术后维持率高，不会引起明显并发症。该研究可能为临床医生使用双极 RF 收紧患者皮肤提供理论指导。
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