Plant Protection Department, Faculty of Agriculture, Assiut University, Assiut, 71526, Egypt.
Department of Biochemistry, College of Sciences, University of Jeddah, 21959, Jeddah, Saudi Arabia.
Sci Rep. 2023 Nov 16;13(1):20009. doi: 10.1038/s41598-023-46231-8.
Deudorix livia (Klug, 1834) (Lepidoptera: Lycaenidae) is one of the most serious lepidopteran insect pests attacking pomegranate fruit around the world, including Egypt (Assiut Governorate, Upper Egypt). To create an effective program (IPM) to control such harmful pests, accurate identification of the pest morphology and genetic structure is essential. Studies on the morphogenetics of this pest are scarce. So, the goal of this research is to identify it both morphologically and genetically. Pomegranate butterfly immature stages were collected from infested pomegranate fruits and reared in the laboratory until the adult's emergence. By using light and scanning electron microscopy, some morphological structures of males and females were studied. DNA was extracted from the legs of a pomegranate butterfly adult. Also, PCR was conducted by using the mitochondrial CO1 gene for sequencing and phylogenetic tests. The results show that the body scales are a mixture of dark and light gray on the dorsal side and white on the ventral side in both sexes. The average male body length (BL) was 11.674 ± 0.299 mm and was 11.458 ± 1.001 mm for the females. The wing venation is similar in both sexes. For the first time, a partial sequence of the mitochondrial CO1 gene in D. livia was deposited in GenBank (MW463927).
石榴蛱蝶（Deudorix livia）（鳞翅目：蛱蝶科）是全世界石榴果实最严重的鳞翅目害虫之一，包括埃及（上埃及的阿斯尤特省）。为了创建一个有效的有害生物综合治理（IPM）计划来控制这种有害生物，准确识别害虫的形态和遗传结构是必不可少的。对这种害虫的形态遗传学研究很少。因此，本研究的目的是对其进行形态学和遗传学鉴定。从受感染的石榴果实中收集石榴蛱蝶幼虫，并在实验室中饲养，直到成虫出现。利用光镜和扫描电子显微镜研究了雌雄成虫的一些形态结构。从石榴蛱蝶成虫的腿中提取 DNA。还使用线粒体 CO1 基因进行 PCR 测序和系统发育测试。结果表明，雌雄体背侧的体鳞片是深灰色和浅灰色的混合物，腹侧是白色的。雄性平均体长（BL）为 11.674±0.299mm，雌性为 11.458±1.001mm。雌雄翅脉相似。首次在石榴蛱蝶的线粒体 CO1 基因的部分序列在 GenBank（MW463927）中被保存。
