Department of Marine Biology and Environmental Sciences, Graduate School of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-Nishi, Miyazaki, Japan.
Department of Marine Biology and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-Nishi, Miyazaki, Japan.
Biosci Biotechnol Biochem. 2024 Jan 24;88(2):206-211. doi: 10.1093/bbb/zbad152.
Paramylon, a starch-like carbohydrate accumulated in Euglena gracilis cells, could be a potential source as a raw material for chemical products; its high-yield production would thus be highly desired. Although the molecular weight and polymerization degree of paramylon are important properties for its use as a raw material for chemical products, the available information about paramylon molecular weight remains insufficient. Therefore, in this study, we investigated a high-density E. gracilis culture approach and how culture conditions affect paramylon molecular weight. The nitrogen source, cultivation temperature, and nutrient feeding were optimized for maximum biomass and paramylon productivity. The maximum dry cell weight and paramylon content yields reached 108.9 g/L and 87.2%, respectively. Paramylon molecular weight was in the range of 220 000-320 000 Da. Our gel permeation chromatography analysis showed that the cells with a higher paramylon content tended to contain paramylon of a higher molecular weight.
拟淀粉是一种在眼虫细胞中积累的淀粉样碳水化合物，它可能是一种有潜力的化学产品原料，因此，人们非常希望能够大量生产它。尽管拟淀粉的分子量和聚合度是其作为化学产品原料的重要性质，但有关拟淀粉分子量的可用信息仍然不足。因此，在这项研究中，我们研究了一种高密度眼虫培养方法，以及培养条件如何影响拟淀粉的分子量。优化了氮源、培养温度和营养物质的供给，以实现最大生物量和拟淀粉生产力。最大干细胞重量和拟淀粉含量产率分别达到 108.9 g/L 和 87.2%。拟淀粉的分子量范围为 220000-320000 Da。我们的凝胶渗透色谱分析表明，具有较高拟淀粉含量的细胞往往含有较高分子量的拟淀粉。
