手性杀菌剂咪鲜胺在四种水果中的立体选择性研究：绝对构型、SFC-MS/MS 对映体分离、降解及风险评估。
Stereoselective study on chiral fungicide metconazole in four kinds of fruits: Absolute configuration, SFC-MS/MS enantioseparation, degradation and risk assessment.
机构信息
College of Food Science & Engineering, Hainan University, No. 158 Renmin Avenue, Haikou 570228, PR China; State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products/Key Laboratory of Detection for Pesticide Residues and Control of Zhejiang, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China; Agricultural Ministry Key Laboratory for Pesticide Residue Detection, Hangzhou 310021, PR China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products/Key Laboratory of Detection for Pesticide Residues and Control of Zhejiang, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China; Agricultural Ministry Key Laboratory for Pesticide Residue Detection, Hangzhou 310021, PR China.
出版信息
Food Chem. 2024 Apr 16;438:137944. doi: 10.1016/j.foodchem.2023.137944. Epub 2023 Nov 7.
Metconazole is a novel chiral fungicide with two chiral carbon atoms, but the research on its stereoselective behavior is limited. Therefore, the stereoselective behaviors of metconazole in four fruits, including grape, peach, pear and jujube, were summarized in this study. After determining the absolute configuration of metconazole stereoisomers, a chiral separation method through supercritical fluid chromatography/tandem triple quadrupole mass spectrometry was first developed, which combined an improved QuEChERS method obtained the recoveries of 71.6-113 % with RSD ≤ 19.8 %. The LOD and LOQ were 4.30-95.9 and 10.5-143.2 ng/kg, respectively. Different stereoselective and diastereoselective behaviors were observed in four fruits. Dietary risk assessments of rac-metconazole were performed in populations with different ages and genders. Both acute (RQ, 0.0124-0.140 %) and chronic (HQ, 0.0234-0.0794 %) intake risks were acceptable. The results of this study would contribute to more complete risk assessments of metconazole and provide data for chiral studies.
咪鲜胺是一种含有两个手性碳原子的新型手性杀菌剂，但关于其立体选择性行为的研究有限。因此，本研究总结了咪鲜胺在葡萄、桃、梨和枣四种水果中的立体选择性行为。在确定咪鲜胺对映异构体的绝对构型后，首次开发了一种通过超临界流体色谱/串联三重四极杆质谱进行手性分离的方法，该方法结合改良的 QuEChERS 方法，回收率为 71.6-113%，RSD≤19.8%。LOD 和 LOQ 分别为 4.30-95.9 和 10.5-143.2ng/kg。在四种水果中观察到不同的立体选择性和非对映选择性行为。对不同年龄和性别的人群进行了 rac-咪鲜胺的膳食风险评估。急性（RQ，0.0124-0.140%）和慢性（HQ，0.0234-0.0794%）摄入风险均可以接受。本研究的结果将有助于更全面地评估咪鲜胺的风险，并为手性研究提供数据。
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