Department of Nephrology, General Hospital of Tianjin Medical University, Tianjin, China.
School of Medical Laboratory, Tianjin Medical University, Tianjin, China.
Clin Rheumatol. 2024 Jan;43(1):307-314. doi: 10.1007/s10067-023-06818-y. Epub 2023 Nov 24.
Thrombocytopenia is one of the primary Sjögren's syndrome (pSS) hematological manifestations. The objective of this study was to evaluate the possible roles of antiplatelet glycoprotein autoantibodies in the pathogenesis of thrombocytopenia in primary Sjögren's syndrome (pSS).
The level of plasma anti-glycoprotein Ib, IIIa and IIb/IIIa autoantibodies in 36 pSS patients without thrombocytopenia and 35 pSS patients with thrombocytopenia, 36 Idiopathic thrombocytopenic purpura (ITP) patients and 39 normal control were measured with enzyme-linked immunosorbent assay (ELISA).
The level of anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies (A490) in the pSS with thrombocytopenia was significantly higher than that of pSS without thrombocytopenia (0.813 ± 0.161 vs 0.688 ± 0.133; 0.917 ± 0.094 vs 0.802 ± 0.070; 0.911 ± 0.125 vs 0.782 ± 0.109). Incidences of the anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies in the pSS with thrombocytopenia was significantly higher than that of pSS without thrombocytopenia (25.7% vs 0%; 65.7% vs 11.1%; 31.4% vs 0%). In patients with pSS, there was a lower platelet count in anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies positive patients ((25.67 ± 5.5) × 10^9/L vs (116.8 ± 84.52) × 10^9/L; 29.04 ± 11.33 × 10^9/L vs (152.0 ± 75.47) × 10^9/L; (31.55 ± 14.0) × 10^9/L vs (118.8 ± 85.24) × 10^9/L).
Elevated plasma levels of anti-platelet glycoprotein autoantibodies may play a role in the pathogenesis of thrombocytopenia in pSS. Key Points • The level of anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies (A490) in the pSS with thrombocytopenia was increased. • Incidences of the anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies in the pSS with thrombocytopenia was increased. • In patients with pSS, there was a lower platelet count in anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies positive patients.
血小板减少症是干燥综合征（pSS）主要的血液学表现之一。本研究旨在评估抗血小板糖蛋白自身抗体在原发性干燥综合征（pSS）血小板减少症发病机制中的可能作用。
采用酶联免疫吸附法（ELISA）检测 36 例无血小板减少的 pSS 患者、35 例血小板减少的 pSS 患者、36 例特发性血小板减少性紫癜（ITP）患者和 39 例正常对照者血浆抗糖蛋白 Ib、IIIa 和 IIb/IIIa 自身抗体水平。
血小板减少的 pSS 患者的抗-GPIb、GPIIIa、GPIIb/IIIa 自身抗体（A490）水平明显高于无血小板减少的 pSS 患者（0.813±0.161 比 0.688±0.133；0.917±0.094 比 0.802±0.070；0.911±0.125 比 0.782±0.109）。血小板减少的 pSS 患者抗-GPIb、GPIIIa、GPIIb/IIIa 自身抗体的发生率明显高于无血小板减少的 pSS 患者（25.7%比 0%；65.7%比 11.1%；31.4%比 0%）。在 pSS 患者中，抗-GPIb、GPIIIa、GPIIb/IIIa 自身抗体阳性患者的血小板计数较低[（25.67±5.5）×10^9/L 比（116.8±84.52）×10^9/L；29.04±11.33×10^9/L 比（152.0±75.47）×10^9/L；（31.55±14.0）×10^9/L 比（118.8±85.24）×10^9/L]。
血小板糖蛋白自身抗体水平升高可能在 pSS 血小板减少症的发病机制中起作用。
血小板减少的 pSS 患者的抗-GPIb、GPIIIa、GPIIb/IIIa 自身抗体（A490）水平升高。
血小板减少的 pSS 患者的抗-GPIb、GPIIIa、GPIIb/IIIa 自身抗体发生率升高。
在 pSS 患者中，抗-GPIb、GPIIIa、GPIIb/IIIa 自身抗体阳性患者的血小板计数较低。
