Kawakubo Y, Liao S K
Anticancer Res. 1986 Sep-Oct;6(5):1197-204.
The effects of bromodeoxyuridine (BUdr) on the in vitro growth, morphology and expression of HLA-DR antigens in the human melanoma line CaCL 73-36 were studied. Treatment of melanoma cells with BUdr resulted in an inhibition of growth with 30-35% reduction in saturation density at 2 micrograms/ml and 50-54% reduction at 4 micrograms/ml. BUdr-treated melanoma cells showed marked morphological changes including more flattened and epithelial cell shapes. These changes are consistent to a less differentiated phenotype. Quantitative absorption analysis using an anti HLA-DR monoclonal antibody revealed that the expression of surface HLA-DR antigens was enhanced by up to 2.5-fold in a BUdr dose-dependent manner. This enhancement could be related to an increased synthesis of HLA-DR antigens rather than a decrease shedding of these antigens. Further studies are needed to fully elucidate the biological significance and mechanism of action of BUdr in causing such altered HLA-DR expression.
研究了溴脱氧尿苷（BUdr）对人黑色素瘤细胞系CaCL 73-36体外生长、形态及HLA-DR抗原表达的影响。用BUdr处理黑色素瘤细胞导致生长受到抑制，在2微克/毫升时饱和密度降低30 - 35%，在4微克/毫升时降低50 - 54%。经BUdr处理的黑色素瘤细胞表现出明显的形态变化，包括更扁平的形态和上皮细胞形状。这些变化与分化程度较低的表型一致。使用抗HLA-DR单克隆抗体进行的定量吸收分析显示，表面HLA-DR抗原的表达以BUdr剂量依赖性方式增强至2.5倍。这种增强可能与HLA-DR抗原合成增加有关，而不是这些抗原的脱落减少。需要进一步研究以充分阐明BUdr导致HLA-DR表达改变的生物学意义和作用机制。
