Lab of Algae Resource Development and Aquaculture Environment Ecological Restoration, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.
Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.
Int J Mol Sci. 2023 Nov 12;24(22):16225. doi: 10.3390/ijms242216225.
, a microalgae species employed for regulating the quality of aquaculture water, demonstrates the capacity to adsorb noxious substances, curtail the growth of detrimental bacteria, and outcompete blooming cyanobacteria. It can be concentrated by natural sedimentation and stored at room temperature, making it costless and simple to transport and use. To study the mechanism of adaptation to room temperature preservation, was concentrated (1.19 × 10-1.21 × 10 cell/mL) and stored for 50 days at low (5 °C, LT), normal (25 °C, NT), and high (35 °C, HT) temperatures, respectively. Polysaccharide content, lipid content, cell survival, and resuscitation were evaluated. RNA-Seq was also used to examine how concentrated responded to temperature. During storage, there was an increase in polysaccharide content and a decrease in lipid content, with both being significantly upregulated in the LT and HT groups. Survival and cell density were highest in the NT group. The RNA-Seq analysis revealed extensive differences in transcript levels. ATP synthesis was inhibited in the LT group due to the reduced expression of , , , , and . Under HT, the formation of reactive oxygen species (ROS) was facilitated by low levels of redox-related genes () and high levels of oxidative genes (, , and . The findings suggest that storing concentrated at room temperature is optimal for microalgae preservation, enhancing theoretical research in this field. Our study provides further theoretical and practical support for the development of as a live ecological preparation for aquaculture microalgae ecology management.
作为一种用于调节水产养殖水质的微藻物种，能够吸附有害物质、抑制有害细菌的生长并与蓝藻竞争。它可以通过自然沉淀浓缩，并在室温下储存，因此运输和使用成本低廉且简单。为了研究适应室温保存的机制，将 （1.19×10-1.21×10 个细胞/毫升）浓缩并分别在低温（5°C，LT）、常温和高温（35°C，HT）下储存 50 天。评估多糖含量、脂含量、细胞存活率和复苏。还使用 RNA-Seq 检查浓缩的 如何响应温度。在储存过程中，多糖含量增加，脂含量减少，LT 和 HT 组均显著上调。NT 组的存活率和细胞密度最高。RNA-Seq 分析显示转录水平存在广泛差异。由于 、 、 、 和 的表达减少，LT 组的 ATP 合成受到抑制。在 HT 下，由于氧化还原相关基因（）水平低和氧化基因（、和 ）水平高，促进了活性氧（ROS）的形成。研究结果表明，室温下储存浓缩的 有利于微藻的保存，为该领域的理论研究提供了支持。本研究为将 作为水产养殖微藻生态学管理的活体生态制剂的开发提供了进一步的理论和实践支持。
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