Department of Bioinspired Science, CHA University, Seongnam-si 13488, Republic of Korea.
PLABiologics Co., Ltd., Seongnam-si 13522, Republic of Korea.
Int J Mol Sci. 2023 Nov 20;24(22):16531. doi: 10.3390/ijms242216531.
The insulin resistance caused by impaired glucose metabolism induces ovarian dysfunction due to the central importance of glucose as a source of energy. However, the research on glucose metabolism in the ovaries is still lacking. The objectives of this study were to analyze the effect of PD-MSCs on glucose metabolism through IGFBP2-AMPK signaling and to investigate the correlation between glucose metabolism and ovarian function. Thioacetamide (TAA) was used to construct a rat injury model. PD-MSCs were transplanted into the tail vein (2 × 10) 8 weeks after the experiment started. The expression of the IGFBP2 gene and glucose metabolism factors (e.g., AMPK, GLUT4) was significantly increased in the PD-MSC group compared to the nontransplantation (NTx) group (* < 0.05). The levels of follicular development markers and the sex hormones AMH, FSH, and E2 were also higher than those in the TAA group. Using ex vivo cocultivation, the mRNA and protein expression of IGFBP2, AMPK, and GLUT4 were significantly increased in the cocultivation with the PD-MSCs group and the recombinant protein-treated group (* < 0.05). These findings suggest that the increased IGFBP2 levels by PD-MSCs play an important role in glucose metabolism and ovarian function through the IGFBP2-AMPK signaling pathway.
葡萄糖代谢受损引起的胰岛素抵抗会导致卵巢功能障碍，因为葡萄糖是能量的重要来源。然而，关于卵巢中葡萄糖代谢的研究仍然缺乏。本研究的目的是分析 PD-MSCs 通过 IGFBP2-AMPK 信号对葡萄糖代谢的影响，并研究葡萄糖代谢与卵巢功能之间的相关性。使用硫代乙酰胺（TAA）构建大鼠损伤模型。实验开始 8 周后，将 PD-MSCs 移植到尾静脉（2×10）8 周。与非移植（NTx）组相比，PD-MSC 组 IGFBP2 基因和葡萄糖代谢因子（如 AMPK、GLUT4）的表达明显增加（<0.05）。卵泡发育标志物和性激素 AMH、FSH 和 E2 的水平也高于 TAA 组。通过体外共培养，与 PD-MSCs 共培养组和重组蛋白处理组的 IGFBP2、AMPK 和 GLUT4 的 mRNA 和蛋白表达明显增加（<0.05）。这些发现表明，PD-MSCs 增加的 IGFBP2 水平通过 IGFBP2-AMPK 信号通路在葡萄糖代谢和卵巢功能中发挥重要作用。
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