Department of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China.
School of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang 550025, China.
Molecules. 2023 Nov 19;28(22):7663. doi: 10.3390/molecules28227663.
Ulcerative colitis (UC) is an intractable disease prevalent worldwide. While ethyl acetate extract from decoction of (EAdSc) has potential anti-inflammatory activity, its effects on UC remain unknown. In this study, the constituent compounds discussed in the literature and identified by gas chromatography and mass spectrometry (GC-MS) were collected, and the blood-soluble components of EAdSc were identified by liquid chromatography-mass spectrometry. The network pharmacology analysis and molecular docking analysis were performed to explore the potential underlying mechanism and active ingredients of EAdSc against UC. Furthermore, mice with dextran sulfate sodium (DSS)-induced UC were used to study the therapeutic effects and validate the mechanism of EAdSc against UC. A total of 53 compounds from EAdSc were identified in the literature and by GC-MS, and 22 blood-soluble EAdSc components were recognized. Network pharmacology analysis revealed that multiple inflammatory signaling pathways are involved in EAdSc's anti-UC activity. Furthermore, molecular docking analysis showed that the eleutheroside A, liriodendrin, epicatechin, 2-methoxy-4-vinylphenol, catechin, androsin, coumaroyltyramine, and catechol may be active against UC through the TLR4/NF-κB/NLRP3 pathway. EAdSc reduced the disease activity, macroscopic colon damage, and histological damage indices, as well as inhibiting DSS-induced spleen enlargement and colon shortening. In addition, EAdSc decreased the levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and IL-17, as well as the expression of TLR4, NF-κB p65, NLRP3, and Caspase-1 mRNA in colon tissues. These results provide insights into the anti-UC effects and underlying mechanisms of EAdSc and help elucidate the active ingredients of EAdSc in the treatment of UC.
溃疡性结肠炎（UC）是一种全球性的难治性疾病。虽然（EAdSc）汤的乙酸乙酯提取物具有潜在的抗炎活性，但它对 UC 的影响尚不清楚。在本研究中，收集了文献中讨论的成分化合物，并通过气相色谱和质谱（GC-MS）进行了鉴定，通过液相色谱-质谱鉴定了 EAdSc 的血溶性成分。通过网络药理学分析和分子对接分析，探讨了 EAdSc 治疗 UC 的潜在机制和活性成分。此外，还使用葡聚糖硫酸钠（DSS）诱导的 UC 小鼠研究了 EAdSc 的治疗效果，并验证了 EAdSc 治疗 UC 的机制。通过 GC-MS 在文献和 GC-MS 中鉴定了 53 种来自 EAdSc 的化合物，鉴定了 22 种血溶性 EAdSc 成分。网络药理学分析表明，多种炎症信号通路参与了 EAdSc 的抗 UC 活性。此外，分子对接分析表明，淫羊藿苷 A、丁香脂素、表儿茶素、2-甲氧基-4-乙烯基苯酚、儿茶素、远志素、咖啡酰酪胺和邻苯二酚可能通过 TLR4/NF-κB/NLRP3 途径对 UC 具有活性。EAdSc 降低了疾病活动度、宏观结肠损伤和组织学损伤指数，抑制了 DSS 诱导的脾脏肿大和结肠缩短。此外，EAdSc 降低了肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β、IL-6 和 IL-17 的水平，以及结肠组织中 TLR4、NF-κB p65、NLRP3 和 Caspase-1 mRNA 的表达。这些结果为 EAdSc 的抗 UC 作用及其潜在机制提供了深入了解，并有助于阐明 EAdSc 治疗 UC 的活性成分。
