BACKGROUND

malaria caused by parasites remains a public health threat. The circumsporozoite proteins (CSPs) of sporozoite play a key role in infection, serving as an excellent vaccine target.

METHODS

using a self-assembled S nanoparticle platform, we generated pseudovirus nanoparticles (PVNPs) displaying CSPs, named S-CSPs, for enhanced immunogenicity.

RESULTS

purified Hisx6-tagged or tag-free S-CSPs self-assembled into PVNPs that consist of a norovirus S inner shell and multiple surface-displayed CSPs. The majority of the PVNPs measured ~27 nm with some size variations, and their three-dimensional structure was modeled. The PVNP-displayed CSPs retained their glycan receptor-binding function. A mouse immunization study showed that PVNPs induced a high antibody response against CSP antigens and the PVNP-immunized mouse sera stained the CSPs of sporozoites at high titer.

CONCLUSIONS AND DISCUSSION

the PVNP-displayed CSPs retain their authentic antigenic feature and receptor-binding function. The CSP-specific antibody elicited by the S-CSP PVNPs binds original CSPs and potentially inhibits the attachment of sporozoites to their host cells, a key step for liver invasion by the sporozoites. Thus, S-CSP PVNPs may be an excellent vaccine candidate against malaria caused by parasites.