含天冬氨酸的热聚氨基酸群体的分级分离及氨基酸组成

Fractionation and amino acid composition of an aspartic acid-containing thermal proteinoid population.

作者信息

Luque-Romero M M, de Medina L S, Blanco J M

出版信息

Biosystems. 1986;19(4):267-72. doi: 10.1016/0303-2647(86)90003-1.

Abstract

The heterogeneity of an aspartic acid-containing thermal polymer population has been studied by anion exchange chromatography, amino acid analysis of the resulting fractions and data processing by the principal components method. By using stepwise or continuous gradients of ionic strength both saw-toothed or bell-shaped elution profiles were obtained. This behaviour and the amino acid composition of analyzed fractions suggest a relatively high degree of heterogeneity in the polymer population although less than theoretically expected. This conclusion is compared with the findings reported in proteinoids made by similar procedures.

摘要

通过阴离子交换色谱法、对所得馏分进行氨基酸分析以及用主成分法进行数据处理，研究了含天冬氨酸热聚合物群体的异质性。通过使用离子强度的逐步或连续梯度，获得了锯齿形或钟形洗脱曲线。这种行为以及分析馏分的氨基酸组成表明，聚合物群体中存在相对较高程度的异质性，尽管低于理论预期。将这一结论与通过类似程序制备的类蛋白中所报道的发现进行了比较。