Institut Cochin, Université de Paris, INSERM U1016, CNRS, F-75014 Paris, France.
Centre de Recherche en Myologie, Sorbonne Université, INSERM UMRS 974, Institut de Myologie, F-75013 Paris, France.
Cells. 2023 Nov 21;12(23):2673. doi: 10.3390/cells12232673.
Satellite cells (SCs) are adult muscle stem cells that are mobilized when muscle homeostasis is perturbed. Here we show that RhoA in SCs is indispensable to have correct muscle regeneration and hypertrophy. In particular, the absence of RhoA in SCs prevents a correct SC fusion both to other RhoA-deleted SCs (regeneration context) and to growing control myofibers (hypertrophy context). We demonstrated that RhoA is dispensable for SCs proliferation and differentiation; however, RhoA-deleted SCs have an inefficient movement even if their cytoskeleton assembly is not altered. Proliferative myoblast and differentiated myotubes without RhoA display a decreased expression of , suggesting a crosstalk between these genes for myoblast fusion regulation. These findings demonstrate the importance of RhoA in SC fusion regulation and its requirement to achieve an efficient skeletal muscle homeostasis restoration.
卫星细胞（SCs）是成体肌肉干细胞，当肌肉内稳态受到干扰时，它们会被动员起来。在这里，我们表明 RhoA 在 SCs 中对于正确的肌肉再生和肥大是必不可少的。具体来说，SC 中 RhoA 的缺失会阻止正确的 SC 融合，无论是在其他 RhoA 缺失的 SC 中（再生情况下）还是在生长中的对照肌纤维中（肥大情况下）。我们证明 RhoA 对于 SC 的增殖和分化是可有可无的；然而，即使它们的细胞骨架组装没有改变，RhoA 缺失的 SCs 的运动效率也会降低。没有 RhoA 的增殖性成肌细胞和分化的肌管显示出下调的 表达，这表明这些基因之间存在着用于成肌细胞融合调节的串扰。这些发现表明 RhoA 在 SC 融合调节中的重要性及其对实现有效的骨骼肌内稳态恢复的必要性。
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