Key Laboratory of Wetland Ecology and Environment, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China.
Key Laboratory of Wetland Ecology and Environment, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Sci Total Environ. 2024 Feb 20;912:169293. doi: 10.1016/j.scitotenv.2023.169293. Epub 2023 Dec 15.
Effects of microplastics (MPs) and nanoplastics (NPs) on the spread of antibiotic resistance genes (ARGs) in soil-plant systems are still unclear. To investigate the spread and mechanisms of ARGs from soil to lettuce, lettuce was exposed to soil spiked with two environmentally relevant concentrations of polystyrene MPs (100 μm) and NPs (100 nm). Results showed that microorganisms that carried ARGs in soil were increased after exposure to MPs/NPs, which led to an increase in ARGs in roots. NPs were absorbed by roots and can be transported to leaves. Analysis of transcriptomics, proteomics and metabolomics indicated that high concentration of NPs regulated the expression of related genes and proteins and improved the accumulation of flavonoids in the lettuce, therefore decreased the abundance of microorganisms that contained ARGs. Our work emphasizes the size and dose influences of MPs and NPs on the spread of ARGs from soil to plant.
微塑料（MPs）和纳米塑料（NPs）对土壤-植物系统中抗生素耐药基因（ARGs）传播的影响尚不清楚。为了研究 ARGs 从土壤向生菜传播的机制和途径，将生菜暴露于两种具有环境相关性浓度的聚苯乙烯 MPs（100μm）和 NPs（100nm）污染的土壤中。结果表明，暴露于 MPs/NPs 后，土壤中携带 ARGs 的微生物增加，导致根中 ARGs 的增加。NPs 被根吸收，并可被运输到叶片。转录组学、蛋白质组学和代谢组学分析表明，高浓度的 NPs 调控了相关基因和蛋白的表达，并提高了生菜中类黄酮的积累，从而降低了含有 ARGs 的微生物的丰度。我们的工作强调了 MPs 和 NPs 的大小和剂量对 ARGs 从土壤向植物传播的影响。
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