Ángel-García Carolina, Rodríguez-Arevalo Kevin Alejandro, Murcia Riaño Nubia, Martínez-Caballero Luz Natalia, Ceballos-Aguirre Germán, Jaramillo Laverde Alejandro, Martínez Mauricio Fernando
Colombian Agricultural Research Corporation-AGROSAVIA, Palmira Research Center, Palmira 763533, Colombia.
Pathogens. 2023 Dec 4;12(12):1418. doi: 10.3390/pathogens12121418.
Hass avocado fruit diseases are one of the main marketing constraints in Colombia. To identify and reveal the diversity of fungi associated with diseases in fruits and peduncles, symptomatic samples were collected from 67 farms in the 9 main Hass avocado-producing municipalities of the department of Cauca located in southwestern Colombia. A total of 237 monosporic isolates were obtained that were subjected to DNA extraction, amplification of the ITS region, sequencing and functional diversity analysis based on Hill numbers by municipality and altitude. The results indicated that the genera , , , , and were associated with fruit diseases. The genus with the highest number of effective species was . The ITS region revealed 100% identity of the species , which was the first report of this pathogen in the crop. There was a greater richness and diversity of associated species in the three municipalities, revealing that the higher the altitude was, the lower the richness and diversity of fungi associated with fruit diseases. These results will provide a better understanding of the identification and diversity of pathogenic microorganisms present in avocado production systems in this region of Colombia.
哈斯牛油果果实病害是哥伦比亚主要的销售限制因素之一。为了识别和揭示与果实及果柄病害相关的真菌多样性，从哥伦比亚西南部考卡省9个主要哈斯牛油果生产市的67个农场采集了有症状的样本。共获得237个单孢分离株，对其进行DNA提取、ITS区域扩增、测序，并基于希尔数按市和海拔进行功能多样性分析。结果表明， 、 、 、 、 和 属与果实病害有关。有效物种数量最多的属是 。ITS区域显示 物种的同一性为100%，这是该病原菌在该作物上的首次报道。三个市中相关物种的丰富度和多样性更高，表明海拔越高，与果实病害相关的真菌丰富度和多样性越低。这些结果将有助于更好地了解哥伦比亚该地区牛油果生产系统中致病微生物的鉴定和多样性。
