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转谷氨酰胺酶对卵清蛋白乳液凝胶作为包埋益生菌载体的影响。

Effect of transglutaminase on ovalbumin emulsion gels as carriers of encapsulated probiotic bacteria.

机构信息

National Research and Development Centre for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China.

Department of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon, South Korea.

出版信息

J Sci Food Agric. 2024 Apr;104(6):3468-3476. doi: 10.1002/jsfa.13232. Epub 2024 Jan 16.

Abstract

BACKGROUND

The use of emulsion gels to protect and deliver probiotics has become an important topic in the food industry. This study used transglutaminase (TGase) to regulate ovalbumin (OVA) to prepare a novel emulsion gel. The effects of OVA concentration and the addition of TGase on the microstructure, rheological properties, water-holding capacity, and stability of the emulsion gels were investigated.

RESULTS

With the addition of TGase and the increasing OVA, the particle size of the emulsion gels decreased significantly (P < 0.05). The gels with TGase exhibited greater water holding, hardness, and chewiness to some extent by forming a more uniform and stable system. After simulated digestion, the survival rate of Bifidobacterium lactis embedded in OVA emulsion gels improved significantly in comparison with the oil-water mixture as a result of the protective effect of the emulsion gel encapsulation.

CONCLUSION

By increasing the OVA content and adding TGase, the rheological characteristics, stability, and encapsulation capability of the OVA emulsion gel could be enhanced, providing a theoretical basis for the use of emulsion gels to construct probiotic delivery systems. © 2023 Society of Chemical Industry.

摘要

背景

利用乳液凝胶来保护和输送益生菌已经成为食品工业的一个重要课题。本研究使用转谷氨酰胺酶(TGase)来调节卵清蛋白(OVA),以制备一种新型乳液凝胶。考察了 OVA 浓度和 TGase 添加量对乳液凝胶的微观结构、流变性能、持水能力和稳定性的影响。

结果

随着 TGase 和 OVA 的添加,乳液凝胶的粒径显著减小(P<0.05)。添加 TGase 的凝胶通过形成更均匀、更稳定的体系,在一定程度上具有更好的持水能力、硬度和咀嚼性。经过模拟消化后,嵌入 OVA 乳液凝胶中的双歧杆菌的存活率与油水混合物相比有了显著提高,这是由于乳液凝胶包埋的保护作用。

结论

通过增加 OVA 含量和添加 TGase,可以增强 OVA 乳液凝胶的流变特性、稳定性和包埋能力,为利用乳液凝胶构建益生菌输送系统提供了理论依据。 © 2023 英国化学学会。

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