[Features of the growth and differentiation of the spinal ganglion of the embryonic rat in diffusion chambers].

Cultivation peculiarities in diffusive chambers on nucleoporous and milliporous filters of the rat embryo spinal ganglia have been investigated on the 18th-20th days of development. Optimal variants have been obtained on the nucleoporous filters. Growth and differentiation of the sensitive neurons and cells of the peripheral glia are realized quicker in the diffusive chambers on the nucleoporous filters, than under in vitro cultivation. In 7-12 days in the diffusive chambers there are already mature pseudounipolar and multipolar neurons, and lemmocyte--axonal interrelations are of definitive character. The disadvantage of the method is its limited time of cultivation, since a connective tissue capsule is formed around the diffusive chamber.