Szafarz D
Biochem Int. 1986 Nov;13(5):735-9.
Formation of crosslinks in DNA by three bifunctional psoralen derivatives plus UVA light in mouse embryo fibroblasts was evaluated by a NaI density gradient centrifugation method. Psoralen was shown to be a more active cross-linking agent than 8-methoxypsoralen. As for 4,5',8-trimethylpsoralen, it needed much lower concentrations and much less 365 nm light fluence to yield high percentages of crosslinked DNA. Repair of adducts formed by these psoralen derivatives was studied by splitting the irradiation dose into two equal parts separated by variously long dark repair periods. It was shown that essentially only monoadducts formed during the first irradiation period were repaired. These mouse embryo fibroblasts seem unable to repair interstrand DNA crosslinks.
采用碘化钠密度梯度离心法评估了三种双功能补骨脂素衍生物加紫外线A光在小鼠胚胎成纤维细胞中诱导DNA交联的情况。结果表明，补骨脂素比8-甲氧基补骨脂素更具活性的交联剂。至于4,5',8-三甲基补骨脂素，它只需更低的浓度和更少的365nm光通量就能产生高比例的交联DNA。通过将照射剂量分成两等份，中间间隔不同时长的暗修复期，研究了这些补骨脂素衍生物形成的加合物的修复情况。结果表明，基本上只有在第一次照射期间形成的单加合物能够被修复。这些小鼠胚胎成纤维细胞似乎无法修复链间DNA交联。
