Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, School of Marine Biology and Fisheries, Hainan University, Haikou, Hainan 570228, China.
Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, School of Marine Biology and Fisheries, Hainan University, Haikou, Hainan 570228, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2024 Mar;49:101184. doi: 10.1016/j.cbd.2023.101184. Epub 2023 Dec 23.
The mud crab (Scylla paramamosain) possesses extensive regenerative abilities, enabling it to replace missing body parts, including claws, legs, and even eyes. Studying the genetic and molecular mechanisms underlying regenerative ability in diverse animal phyla has the potential to provide new insights into regenerative medicine in humans. In the present study, we performed mRNA sequencing to reveal the genetic mechanisms underlying the claw regeneration in mud crab. Several differentially expressed genes (DEGs) were expressed in biological pathways associated with cuticle synthase, collagen synthase, tissue regeneration, blastema formation, wound healing, cell cycle, cell division, and cell migration. The top GO enrichment terms were microtubule-based process, collagen trimer, cell cycle process, and extracellular matrix structural constituent. The most enriched KEGG pathways were ECM-receptor interaction and focal adhesion. The genes encoding key functional proteins, such as collagen alpha, cuticle protein, early cuticle protein, arthrodial cuticle protein, dentin sialophosphoprotein (DSPP), epidermal growth factor receptor (EGFR), kinesin family member C1 (KIFC1), and DNA replication licensing factor mcm2-like (MCM2) were the most significant and important DEGs suspected to participate in claw regeneration. The findings of this research offer a comprehensive and insightful understanding of the genetic and molecular mechanisms underlying claw regeneration in S. paramamosain. By elucidating the specific genes and molecular pathways implicated in this process, our study contributes significantly to the broader field of regenerative biology and offers potential avenues for further exploration in crustacean limb regeneration.
泥蟹（Scylla paramamosain）具有广泛的再生能力，能够替换缺失的身体部位，包括爪子、腿，甚至眼睛。研究不同动物门中再生能力的遗传和分子机制，有可能为人类再生医学提供新的见解。在本研究中，我们进行了 mRNA 测序，以揭示泥蟹爪再生的遗传机制。在与角质层合成酶、胶原合成酶、组织再生、芽基形成、伤口愈合、细胞周期、细胞分裂和细胞迁移相关的生物途径中表达了几个差异表达基因 (DEG)。GO 富集分析的顶级术语是微管为基础的过程、胶原三聚体、细胞周期过程和细胞外基质结构成分。最富集的 KEGG 途径是细胞外基质受体相互作用和焦点黏附。编码关键功能蛋白的基因，如胶原 alpha、角质层蛋白、早期角质层蛋白、关节角质层蛋白、牙本质涎磷蛋白 (DSPP)、表皮生长因子受体 (EGFR)、驱动蛋白家族成员 C1 (KIFC1) 和 DNA 复制许可因子 mcm2 样 (MCM2)，是最显著和重要的差异表达基因，推测它们参与了爪的再生。这项研究的结果为 S. paramamosain 爪再生的遗传和分子机制提供了全面而深入的理解。通过阐明涉及这个过程的特定基因和分子途径，我们的研究为更广泛的再生生物学领域做出了重要贡献，并为甲壳类动物肢体再生的进一步探索提供了潜在途径。
