Yonemura S, Mabuchi I
Cell Motil Cytoskeleton. 1987;7(1):46-53. doi: 10.1002/cm.970070107.
The distribution of actin filaments in the cortical layer of sea urchin eggs during fertilization has been investigated by light microscopy using fluorescently labeled phallotoxins. The cortical layer of both whole eggs and cortices isolated on a glass surface was examined. In cortices of unfertilized eggs, numerous fluorescent spots were seen, which may correspond to short actin filament cores in microvilli. After insemination, one of the sperm-attaching points on the egg surface first became strongly fluorescent. This fluorescence grew around the point of sperm penetration with the growth of the fertilization cone. Then, the cortical layer of the egg around the fertilization cone became strongly fluorescent and the fluorescence propagated in a wavelike manner over the entire cortex. The mechanism of the propagation of actin polymerization is discussed.
利用荧光标记的鬼笔环肽通过光学显微镜研究了受精过程中海胆卵皮质层中肌动蛋白丝的分布。对完整卵的皮质层以及分离在玻璃表面上的皮质层都进行了检查。在未受精卵的皮质层中，可以看到许多荧光斑点，这些斑点可能对应于微绒毛中的短肌动蛋白丝核心。授精后，卵表面上的一个精子附着点首先变得强烈荧光化。随着受精锥的生长，这种荧光在精子穿透点周围增强。然后，受精锥周围的卵皮质层变得强烈荧光化，并且荧光以波状方式在整个皮质层中传播。本文讨论了肌动蛋白聚合传播的机制。
