Gaur Kajal, Varshney Himanshi, Subhan Iqra, Fatima Javeria, Jyoti Smita, Siddique Yasir Hasan
Laboratory of Alternative Animal Models, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India.
Department of Zoology, School of Sciences, IFTM University, Moradabad, UP, India.
Food Chem Toxicol. 2024 Feb;184:114425. doi: 10.1016/j.fct.2023.114425. Epub 2023 Dec 29.
Bis(2-ethylhexyl) phthalate, generally known as DEHP is a synthetic compound mainly used as a plasticizer to make polyvinyl chloride products flexible and soft. The present work aimed to study the toxicity of Bis(2-ethylhexyl) phthalate on the third instar larvae of transgenic Drosophila melanogaster(hsp70-lacZ) Bg. The hsp70 gene is associated with the β-galactosidase in our present transgenic strain therefore, the more activity of β-galactosidase will indirectly correspond to hsp70 expression. The third instar larvae were allowed to feed on the diet for 24 h having 0.001, 0.005, 0.01, and 0.02 M of Bis(2-ethylhexyl) phthalate at the final concentration. After the exposure of 24hrs, the larvae were subjected to ONPG assay, X-gal staining, trypan blue exclusion test, oxidative stress markers assays, and comet assay. A dose-dependent increase in hsp70 expression, tissue damage, Glutathione-S-transferase (GST) activity, lipid peroxidation, monoamine oxidase, caspase-9 & 3, protein carbonyl content (PCC), DNA damage and decrease in the glutathione (GSH) content, delta-aminolevulinic acid dehydrogenase (ẟ-ALD-D) and acetylcholinesterase activity were observed in the larvae exposed to 0.005, 0.01, 0.02 M of Bis-(2-ethylhexyl) phthalate. The dose of 0.001 M of Bis(2-ethylhexyl) phthalate did not showed any toxic effects and hence can be considered as No Observed Adverse Effect Level (NOAEL) for Bis(2-ethylhexyl) phthalate. The study supports the use of Drosophila for the evaluation of possible toxic effects associated with synthetic compounds.
邻苯二甲酸二（2-乙基己基）酯，通常称为DEHP，是一种合成化合物，主要用作增塑剂以使聚氯乙烯产品具有柔韧性和柔软性。本研究旨在探讨邻苯二甲酸二（2-乙基己基）酯对转基因黑腹果蝇（hsp70-lacZ）Bg三龄幼虫的毒性。在我们目前的转基因品系中，hsp70基因与β-半乳糖苷酶相关，因此，β-半乳糖苷酶的活性越高，将间接对应于hsp70的表达。让三龄幼虫取食最终浓度分别为0.001、0.005、0.01和0.02 M邻苯二甲酸二（2-乙基己基）酯的饲料24小时。暴露24小时后，对幼虫进行ONPG测定、X-gal染色、台盼蓝排斥试验、氧化应激标志物测定和彗星试验。在暴露于0.005、0.01、0.02 M邻苯二甲酸二（2-乙基己基）酯的幼虫中，观察到hsp70表达、组织损伤、谷胱甘肽-S-转移酶（GST）活性、脂质过氧化、单胺氧化酶、半胱天冬酶-9和3、蛋白质羰基含量（PCC）、DNA损伤呈剂量依赖性增加，而谷胱甘肽（GSH）含量、δ-氨基乙酰丙酸脱氢酶（δ-ALD-D）和乙酰胆碱酯酶活性降低。0.001 M邻苯二甲酸二（2-乙基己基）酯剂量未显示任何毒性作用，因此可被视为邻苯二甲酸二（2-乙基己基）酯的未观察到有害作用水平（NOAEL）。该研究支持使用果蝇来评估与合成化合物相关的可能毒性作用。
