Green S T
Clin Sci (Lond). 1987 Feb;72(2):233-8. doi: 10.1042/cs0720233.
The role of the autonomic nervous system in the control of thyroxine release from the rat thyroid gland in vitro, has been investigated using electrical field stimulation as a device to induce intrathyroidal nerves to release their neurotransmitters. The effects of field stimulation on follicular cell membrane potential and input resistance have been investigated using continuous intracellular recordings from individual cells. Electrical field stimulation promotes up to an eightfold increase in throxine release from the thyroid gland in vitro. This effect is blocked by the neurotoxin, tetrodotoxin. Electrical field stimulation has no effect on follicular cell membrane potential or input resistance. This is consistent with the results of previous pharmacological experiments using this preparation. It is concluded that electrical field stimulation induces intrathyroidal nerves to release their neurotransmitters, and that the net effect of this is to cause thyroxine release from the thyroid follicles. The existence of a directly acting secretomotor innervation to the thyroid follicle is suggested.
利用电场刺激作为诱导甲状腺内神经释放神经递质的手段，对自主神经系统在体外控制大鼠甲状腺释放甲状腺素中的作用进行了研究。通过对单个细胞进行连续细胞内记录，研究了电场刺激对滤泡细胞膜电位和输入电阻的影响。电场刺激可使体外甲状腺的甲状腺素释放增加高达八倍。这种效应可被神经毒素河豚毒素阻断。电场刺激对滤泡细胞膜电位或输入电阻没有影响。这与使用该制剂的先前药理学实验结果一致。得出的结论是，电场刺激诱导甲状腺内神经释放其神经递质，其净效应是导致甲状腺滤泡释放甲状腺素。提示存在对甲状腺滤泡的直接作用的分泌运动神经支配。
