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针对铜绿微囊藻的 H6 特性。

The characteristics of H6 against Microcystis aeruginosa.

机构信息

School of Environment and Resources, Southwest University of Science and Technology, Mianyang, 621010, China.

College of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.

出版信息

Environ Sci Pollut Res Int. 2024 Jan;31(5):7702-7711. doi: 10.1007/s11356-023-31616-z. Epub 2024 Jan 3.

Abstract

Algal bloom caused by Microcystis aeruginosa has always been the focus of attention; microbial algal control has the advantages of significant effect, low investment cost, and environmental friendliness; the use of microbial technology to inhibit the bloom has a broad prospect for development. In this study, a strain of Enterobacterium algicidal bacteria screened from a river was used to study the algicidal characteristics against Microcystis aeruginosa using SEM, 3-D EEM and zeta potential. The results showed that the optimal dosage (v/v) of the strain was 5% and the removal rate of algal cells was 70% after 7 days. When the algal density was OD = 0.3, the removal rate of algal cells reached 83% after 7 days. In the pH range of 5 ~ 11, the removal rate of algal cells was 70 ~ 80% after 7 days. Algicidal bacteria H6 is mainly indirect algae lysis and is supplemented by direct algae lysis. Algicidal bacteria H6 removes algicidal substances by secreting high temperature resistant algicidal substances and algicidal products are humic acids. Algicidal bacterium H6 was a strain of Enterobacterium with good algicidal effect in a wide pH range, which enriched the bacterial resources in the control of cyanobacteria bloom in water. The high temperature resistance of the algae-soluble substance secreted by the algae-soluble substance provided convenience for the subsequent preparation and application of bacterial powder.

摘要

铜绿微囊藻引发的水华一直是关注的焦点;微生物抑藻具有效果显著、投资成本低、环境友好等优点;利用微生物技术抑制藻类的爆发具有广阔的发展前景。本研究从河流中筛选出一株肠杆菌杀藻菌,采用 SEM、3-D EEM 和 ζ 电位等方法研究其对铜绿微囊藻的杀藻特性。结果表明,该菌株的最佳投加量(v/v)为 5%,7 天后藻细胞去除率为 70%。当藻密度 OD=0.3 时,7 天后藻细胞去除率达到 83%。在 pH 值为 511 的范围内,7 天后藻细胞的去除率为 70%80%。杀藻菌 H6 主要通过间接裂解藻类,辅以直接裂解藻类。杀藻菌 H6 通过分泌耐高温的杀藻物质来去除杀藻物质,杀藻产物是腐殖酸。杀藻菌 H6 是一株在较宽 pH 值范围内具有良好杀藻效果的肠杆菌,丰富了水中蓝藻水华控制的细菌资源。杀藻物质分泌的高温耐受物质为后续细菌粉的制备和应用提供了便利。

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