使用pH可切换的深共熔溶剂和HPLC-UV分析法灵敏测定白血病患儿血浆中的柔红霉素。
Sensitive determination of daunorubicin in plasma of children with leukemia using pH-switchable deep eutectic solvents and HPLC-UV analysis.
作者信息
Akramipour Reza, Babaei Homa, Castru-Cayllaha Fiedel, Golpayegani Mohammad Reza, Fattahi Nazir, Fattahi Farshad
机构信息
School of Medical, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Clinical Research Development Center, Imam Khomeini and Mohammad Kermanshahi and Farabi Hospitals, Kermanshah University of Medical Sciences, Kermanshah, Iran.
出版信息
Heliyon. 2023 Dec 10;10(1):e23532. doi: 10.1016/j.heliyon.2023.e23532. eCollection 2024 Jan 15.
An environmental friendly, fast, easy and inexpensive liquid-liquid microextraction (LLME) in combination with pH-switchable deep eutectic solvent (DES) method followed by HPLC was investigated for the separation and determination of daunorubicin (DNR) in human plasma samples. For this purpose, first, 9 DESs were prepared based on previous studies and their switchability in aqueous solution was evaluated by changing the pH. Non-switchable DESs were discarded and switchable DESs were used to extract DNR. The parameters affecting the extraction efficiency were optimized (DES type, volume of DES, concentration of KOH, volume of HCl, salt addition and extraction time). After optimizing the conditions and drawing the calibration curve, figures of merit were calculated. Relative standard deviations (%RSDs) based on 7 replicate with 50 μg L of DNR in plasma were 2.7 for intra-day and 4.8 % for inter-day. A wide linear range from 0.15 to 200 μg L was obtained. The detection limit of the method based on signal-to-noise 3 and the quantification limit of the method based on signal-to-noise 10 were 0.05 and 0.15, respectively. After spiking plasma samples with different concentrations of DNR, relative recoveries were obtained in the range of 91.0-107.8 %.
研究了一种环境友好、快速、简便且廉价的液-液微萃取（LLME）结合pH可切换的深共熔溶剂（DES）方法，随后采用高效液相色谱法（HPLC）对人血浆样品中的柔红霉素（DNR）进行分离和测定。为此，首先根据先前的研究制备了9种DES，并通过改变pH值评估其在水溶液中的可切换性。不可切换的DES被丢弃，可切换的DES用于萃取DNR。对影响萃取效率的参数进行了优化（DES类型、DES体积、KOH浓度、HCl体积、加盐量和萃取时间）。在优化条件并绘制校准曲线后，计算了方法的性能指标。在血浆中加入50 μg/L的DNR进行7次重复测定，日内相对标准偏差（%RSD）为2.7%，日间为4.8%。获得了0.15至200 μg/L的宽线性范围。基于信噪比为3的方法检测限和基于信噪比为10的方法定量限分别为0.05和0.15。在血浆样品中加入不同浓度的DNR后，相对回收率在91.0%至107.8%之间。
Zotero 插件