Dicle Yalcin, Aydin Elif, Seker Ugur
Department of Medical Microbiology, Faculty of Medicine, Mardin Artuklu University, 47200, Mardin, Türkiye.
Tavsanli Vocational School of Health Services, Kutahya Health Sciences University, 43300, Kutahya, Türkiye.
Toxicol Res (Camb). 2023 Dec 5;13(1):tfad112. doi: 10.1093/toxres/tfad112. eCollection 2024 Feb.
In the present study, a cecal ligation and puncture (CLP)-induced experimental sepsis rat model was used to explore the effects of baicalein on inflammatory cytokine levels and oxidative stress as well as the possible regulatory role of nuclear factor-kappa B (NF-κB).
For that purpose, 42 Wistar albino rats were equally divided into control, sham, sepsis, B50 + S, B100 + S, S + B50, and S + B100 groups. The B50 + S and B100 + S groups received baicalein before the induction of sepsis, while the S + B50 and S + B100 groups received baicalein afterwards. Experimental sepsis in related groups is generated through ligation of cecum and a puncture in cecal wall. Serum samples were used for tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) analyses, and tissue Malondialdehyde (MDA), Superoxide dismutase (SOD), Glutathione (GSH), IL-6, and NF-κB levels were measured.
Compared to the control group, there were significantly increases in the serum TNF-α, IL-6, tissue MDA, and NF-κB levels and decreases in the tissue SOD and GSH levels in the septic group ( < 0.05). Compared to the septic group, inflammation and oxidative stress were reduced in the baicalein-treated groups. Although all of the pre- and post-treatment protocols alleviated inflammation and oxidative stress to varying degrees, pre-treatment with 100 mg/kg was the most successful.
Findings of this study indicated that baicalein has the potential to reduce sepsis-related oxidative stress and inflammation in the lungs and that pathological outcomes could be regulated via NF-κB transcription factor activity.
在本研究中，采用盲肠结扎和穿刺（CLP）诱导的实验性脓毒症大鼠模型，以探讨黄芩苷对炎性细胞因子水平和氧化应激的影响以及核因子-κB（NF-κB）可能的调节作用。
为此，将42只Wistar白化大鼠平均分为对照组、假手术组、脓毒症组、B50 + S组、B100 + S组、S + B50组和S + B100组。B50 + S组和B100 + S组在诱导脓毒症前给予黄芩苷，而S + B50组和S + B100组在诱导脓毒症后给予黄芩苷。相关组的实验性脓毒症通过盲肠结扎和盲肠壁穿刺产生。采集血清样本用于分析肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6），并测定组织丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、IL-6和NF-κB水平。
与对照组相比，脓毒症组血清TNF-α、IL-6、组织MDA和NF-κB水平显著升高，组织SOD和GSH水平降低（P < 0.05）。与脓毒症组相比，黄芩苷治疗组的炎症和氧化应激减轻。尽管所有的预处理和后处理方案均不同程度地减轻了炎症和氧化应激，但100 mg/kg的预处理最为成功。
本研究结果表明，黄芩苷具有减轻脓毒症相关的肺部氧化应激和炎症的潜力，并且病理结果可通过NF-κB转录因子活性进行调节。
