The Third Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210028, China; Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China.
The Third Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210028, China; Key Laboratory of New Drug Delivery Systems of Chinese Materia Medica, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.
J Pharm Biomed Anal. 2024 Mar 15;240:115957. doi: 10.1016/j.jpba.2023.115957. Epub 2023 Dec 30.
Epimedium is a Chinese herbal medicine commonly used in clinical practice to reinforce yang. Previous studies have shown that Epimedium fried with suet oil based has the best effect on warming kidney and promoting yang. Evidence suggests a relationship between kidney yang deficiency syndrome (KYDS) and metabolic disorders of the intestinal microflora. However, the specific interaction between KYDS and the intestinal microbiome, as well as the internal regulatory mechanism of the KYDS intestinal microbiome regulated by Epimedium fried with suet oil, remain unclear. The purpose of this study was to investigate the regulatory effects of different processed products of Epimedium on intestinal microflora and metabolites in rats with kidney yang deficiency, and to reveal the processing mechanism of Epimedium fried with suet oil warming kidney and helping yang. 16 S rRNA and LC-MS/MS technology were used to detect fecal samples. Combined with multivariate statistical analysis, differential intestinal flora and metabolites were screened. Then the content of differential bacteria was then quantified using quantitative real-time fluorescence PCR. Furthermore, the correlation between differential bacterial flora and metabolites was analyzed using Spearman's method. The study found that the composition of intestinal flora in rats with kidney yang deficiency changed compared to healthy rats. Epimedium fried with suet oil could increase the levels of beneficial bacteria, while significantly reducing the levels of harmful bacteria. Real-time quantitative PCR results were consistent with 16 S rRNA gene sequencing analysis. Fecal metabolomics revealed that KYDS was associated with 30 different metabolites, involving metabolic pathways steroid hormone biosynthesis etc. Moreover, differential bacteria were closely correlated with potential biomarkers. Epimedium could improve metabolic disorders associated with KYDS by acting on the intestinal flora, with Epimedium fried with suet oil demonstrating the most effective regulatory effect. Its potential mechanism may involve the regulation of abnormal metabolism and the impact on the diversity and structure of the intestinal flora.
淫羊藿是一种常用于临床实践以壮阳的中药。先前的研究表明，以羊脂油炮制的淫羊藿对温肾壮阳的效果最佳。有证据表明，肾阳虚证（KYDS）与肠道微生物群的代谢紊乱之间存在关联。然而，KYDS 与肠道微生物群之间的具体相互作用，以及淫羊藿羊脂油炮制物调节 KYDS 肠道微生物群的内在调节机制尚不清楚。本研究旨在探讨不同炮制的淫羊藿对肾阳虚大鼠肠道微生物群和代谢物的调节作用，揭示淫羊藿羊脂油炮制温肾助阳的炮制机制。16S rRNA 和 LC-MS/MS 技术用于检测粪便样本。结合多变量统计分析，筛选出差异肠道菌群和代谢物。然后使用定量实时荧光 PCR 定量差异细菌的含量。此外，还使用 Spearman 方法分析了差异细菌菌群与代谢物之间的相关性。研究发现，与健康大鼠相比，肾阳虚大鼠的肠道菌群组成发生了变化。羊脂油炮制的淫羊藿可以增加有益菌的水平，同时显著降低有害菌的水平。实时定量 PCR 结果与 16S rRNA 基因测序分析一致。粪便代谢组学揭示 KYDS 与 30 种不同的代谢物有关，涉及类固醇激素生物合成等代谢途径。此外，差异细菌与潜在生物标志物密切相关。淫羊藿可以通过作用于肠道菌群来改善与 KYDS 相关的代谢紊乱，其中以羊脂油炮制的淫羊藿的调节作用最为有效。其潜在机制可能涉及对异常代谢的调节以及对肠道菌群多样性和结构的影响。
Zhongguo Zhong Yao Za Zhi. 2014-2
J Chromatogr B Analyt Technol Biomed Life Sci. 2023-2-1
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