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红茶蒸牛肉的品质分析及红茶主要活性成分对肌原纤维蛋白作用机制的研究

Quality analysis of steamed beef with black tea and the mechanism of action of main active ingredients of black tea on myofibrillar protein.

机构信息

College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, Shaanxi, China.

College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, Shaanxi, China.

出版信息

Food Chem. 2024 May 30;441:137997. doi: 10.1016/j.foodchem.2023.137997. Epub 2023 Nov 14.

Abstract

In this study, we analyzed the tea polyphenol composition, volatile flavor composition and storage stability of steamed beef with black tea. The molecular docking and dynamics were used to elucidate the interaction mechanism between the active components of black tea and myofibrillar proteins. The highest content of caffeine (CAF) was found in black tea steamed beef products, followed by catechin (C), epicatechin gallate (ECG), epicatechin gallate (EGCG) and theaflavins (TF). Steamed beef with black tea showed low ΔE* value, low TBARS value, low carbonyl content as well as high sulfhydryl content during storage. The addition of C, CAF, ECG, EGCG and TF enhanced the oxidative stability of myofibrillar protein. In this study, the effects of active components of black tea on the oxidative stability of myofibrillar protein and their interactions were determined, which could provide a reference for the application of black tea and its active components in meat products. At the same time, it can provide new ideas for the development of new meat products.

摘要

本研究分析了红茶蒸牛肉中的茶多酚组成、挥发性风味成分和贮藏稳定性。采用分子对接和动力学方法阐明了红茶活性成分与肌原纤维蛋白的相互作用机制。红茶蒸牛肉制品中咖啡因(CAF)含量最高,其次是儿茶素(C)、表儿茶素没食子酸酯(ECG)、表没食子儿茶素没食子酸酯(EGCG)和茶黄素(TF)。在贮藏过程中,红茶蒸牛肉的 ΔE* 值较低,TBARS 值、羰基含量较低,巯基含量较高。C、CAF、ECG、EGCG 和 TF 的添加提高了肌原纤维蛋白的氧化稳定性。本研究确定了红茶活性成分对肌原纤维蛋白氧化稳定性的影响及其相互作用,可为红茶及其活性成分在肉制品中的应用提供参考。同时,为开发新型肉类产品提供了新的思路。

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