Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, College of Geography and Oceanography, Minjiang University, Fuzhou, 350108, China.
SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, China.
Aquat Toxicol. 2024 Feb;267:106813. doi: 10.1016/j.aquatox.2023.106813. Epub 2023 Dec 21.
Nanoplastics can interact with antibiotics, altering their bioavailability and the ensuing toxicity in marine organisms. It is reported that plain polystyrene (PS) nanoplastics decrease the bioavailability and adverse effects of sulfamethazine (SMZ) on the gut microbiota in Oryzias melastigma. However, the influence of surface functional groups on the combined effects with SMZ remains largely unknown. In this study, adult O. melastigma were fed diet amended with 4.62 mg/g SMZ and 3.65 mg/g nanoplastics (i.e., plain PS, PS-COOH and PS-NH) for 30 days (F0-E), followed by a depuration period of 21 days (F0-D). In addition, the eggs produced on the last day of exposure were cultured under standard protocols without further exposure for 2 months (F1 fish). The results showed that the alpha diversity or the bacterial community of gut microbiota did not differ among the SMZ + PS, SMZ + PS-COOH, and SMZ + PS-NH groups in the F0-E and F1 fish. Interestingly, during the depuration, a clear recovery of gut microbiota (e.g., increases in the alpha diversity, beneficial bacteria abundances and network complexity) was found in the SMZ + PS group, but not for the SMZ + PS-COOH and SMZ + PS-NH groups, indicating that PS-COOH and PS-NH could prolong the toxic effect of SMZ and hinder the recovery of gut microbiota. Compared to plain PS, lower egestion rates of PS-COOH and PS-NH were observed in O. melastigma. In addition, under the simulated fish digest conditions, the SMZ-loaded PS-NH was found to desorb more SMZ than the loaded PS and PS-COOH. These results suggested that the surface -COOH and -NH groups on PS could influence their egestion efficiency and the adsorption/desorption behavior with SMZ, resulting in a long-lasting SMZ stress in the gut during the depuration phase. Our findings highlight the complexity of the carrier effect and ecological risk of surface-charged nanoplastics and the interactions between nanoplastics and antibiotics in natural environments.
纳米塑料可以与抗生素相互作用，改变其在海洋生物体内的生物可利用性和随之产生的毒性。据报道，普通聚苯乙烯（PS）纳米塑料降低了磺胺甲恶唑（SMZ）在泥鳅肠道微生物群中的生物可利用性和不良影响。然而，表面官能团对与 SMZ 联合作用的影响在很大程度上尚不清楚。在这项研究中，成年泥鳅被喂食添加了 4.62 mg/g SMZ 和 3.65 mg/g 纳米塑料（即普通 PS、PS-COOH 和 PS-NH）的饮食 30 天（F0-E），然后进行 21 天的净化期（F0-D）。此外，在暴露的最后一天产生的卵在没有进一步暴露的情况下按照标准方案培养 2 个月（F1 鱼）。结果表明，在 F0-E 和 F1 鱼中，SMZ+PS、SMZ+PS-COOH 和 SMZ+PS-NH 组之间的肠道微生物群的 alpha 多样性或细菌群落没有差异。有趣的是，在净化过程中，SMZ+PS 组的肠道微生物群明显恢复（例如，alpha 多样性增加、有益细菌丰度增加和网络复杂性增加），但 SMZ+PS-COOH 和 SMZ+PS-NH 组没有，表明 PS-COOH 和 PS-NH 可以延长 SMZ 的毒性作用并阻碍肠道微生物群的恢复。与普通 PS 相比，在泥鳅中观察到 PS-COOH 和 PS-NH 的排泄率较低。此外，在模拟鱼类消化条件下，负载 SMZ 的 PS-NH 被发现比负载 PS 和 PS-COOH 的 SMZ 解吸更多。这些结果表明，PS 上的表面-COOH 和-NH 基团可以影响它们的排泄效率以及与 SMZ 的吸附/解吸行为，从而在净化阶段导致肠道中持续存在 SMZ 压力。我们的研究结果强调了载体效应的复杂性和表面带电纳米塑料的生态风险，以及自然环境中纳米塑料和抗生素之间的相互作用。
Front Toxicol. 2025-5-30
Zotero 插件