雄性和雌性大鼠垂体中生长激素细胞和催乳素细胞的出生后发育。免疫细胞化学光镜和电镜研究。

Postnatal development of growth hormone and prolactin cells in male and female rat pituitary. An immunocytochemical light and electron microscopic study.

作者信息

Smets G, Velkeniers B, Finne E, Baldys A, Gepts W, Vanhaelst L

出版信息

J Histochem Cytochem. 1987 Mar;35(3):335-41. doi: 10.1177/35.3.3819376.

DOI:10.1177/35.3.3819376

PMID:3819376

Abstract

Localization and ultrastructural maturation of prolactin (PRL) and growth hormone (GH) cells were studied in pituitaries from neonatal, immature (4-6 weeks old), and adult rats (2-3 months old) by light and electron microscopic immunocytochemistry. The distribution pattern of these cells did not change with age. Both cell types were concentrated laterodorsally, with PRL cells adjacent to the intermediate lobe and GH cells nearer the center of the pars distalis. Labeling density of the immunogold reaction was highest for both hormones in immature rats. In neonatal and immature rats, one PRL cell type with granules 200 nm in diameter was present. In adult rats, two types of PRL cells were present: one containing polymorphous granules measuring about 500 nm (prevalent in female rats), the other with spherical granules about 200 nm (prevalent in male rats). No changes were detected in GH cells during maturation.

摘要

通过光镜和电镜免疫细胞化学方法，研究了新生、未成熟（4 - 6周龄）和成年大鼠（2 - 3月龄）垂体中催乳素（PRL）细胞和生长激素（GH）细胞的定位及超微结构成熟情况。这些细胞的分布模式不随年龄变化。两种细胞类型均集中于背外侧，PRL细胞靠近中间叶，GH细胞更靠近远侧部中心。未成熟大鼠中两种激素的免疫金反应标记密度最高。在新生和未成熟大鼠中，存在一种直径为200 nm颗粒的PRL细胞类型。成年大鼠中存在两种PRL细胞类型：一种含有直径约500 nm的多形性颗粒（在雌性大鼠中占优势），另一种含有直径约200 nm的球形颗粒（在雄性大鼠中占优势）。成熟过程中GH细胞未检测到变化。