McKinley Endya, Speckhart Savannah L, Keane Jessica A, Oliver Mary A, Rhoads Michelle L, Edwards J Lannett, Biase Fernando H, Ealy Alan D
School of Animal Sciences, Virginia Tech, Blacksburg, VA 24061, USA.
Department of Animal Science, The University of Tennessee, Knoxville, TN 37996, USA.
Animals (Basel). 2023 Dec 21;14(1):44. doi: 10.3390/ani14010044.
This work explored whether supplementing selective members of the interleukin-6 (IL6) cytokine family during in vitro bovine oocyte maturation affects maturation success, cumulus-oocyte complex (COC) gene expression, fertilization success, and embryo development potential. Human recombinant proteins for IL6, IL11, and leukemia inhibitory factor (LIF) were supplemented to COCs during the maturation period, then fertilization and embryo culture commenced without further cytokine supplementation. The first study determined that none of these cytokines influenced the rate that oocytes achieved arrest at meiosis II. The second study identified that LIF and IL11 supplementation increases transcript abundance. Supplementation with IL6 supplementation did not affect abundance but reduced transcript abundance. Several other transcriptional markers of oocyte competency were not affected by any of the cytokines. The third study determined that supplementing these cytokines during maturation did not influence fertilization success, but either LIF or IL11 supplementation increased blastocyst development. No effect of IL6 supplementation on subsequent blastocyst development was detected. The fourth experiment explored whether each cytokine treatment affects the post-thaw survivability of cryopreserved IVP blastocysts. None of the cytokines supplemented during oocyte maturation produced any positive effects on post-thaw blastocyst re-expansion and hatching. In conclusion, these outcomes implicate IL11 and LIF as potentially useful supplements for improving bovine oocyte competency.
本研究探讨了在体外牛卵母细胞成熟过程中补充白细胞介素-6（IL6）细胞因子家族的特定成员是否会影响成熟成功率、卵丘-卵母细胞复合体（COC）基因表达、受精成功率和胚胎发育潜力。在成熟期间向COC中补充人重组IL6、IL11和白血病抑制因子（LIF）蛋白，然后在不进一步补充细胞因子的情况下开始受精和胚胎培养。第一项研究确定这些细胞因子均未影响卵母细胞在减数分裂II期停滞的速率。第二项研究发现补充LIF和IL11会增加转录本丰度。补充IL6不影响丰度，但会降低转录本丰度。卵母细胞能力的其他几个转录标记不受任何细胞因子的影响。第三项研究确定在成熟过程中补充这些细胞因子不会影响受精成功率，但补充LIF或IL11会增加囊胚发育。未检测到补充IL6对后续囊胚发育的影响。第四个实验探讨了每种细胞因子处理是否会影响冷冻保存的体外受精囊胚解冻后的存活率。卵母细胞成熟过程中补充的细胞因子均未对解冻后囊胚的再扩张和孵化产生任何积极影响。总之，这些结果表明IL11和LIF可能是提高牛卵母细胞能力的有用补充剂。
