Chanchi Prashanthkumar Mallikarjun, Temdee Wattana, Mittal Ajay, Suyapoh Watcharapol, Sornying Peerapon, Palamae Suriya, Bautong Jirayu, Zhang Bin, Hong Hui, Benjakul Soottawat
International Center of Excellence in Seafood Science and Innovation, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai 90110, Thailand.
Veterinary Pathology Unit, Department of Veterinary Science, Faculty of Veterinary Science, Prince of Songkla University, Hat Yai 90110, Thailand.
Foods. 2023 Dec 20;13(1):28. doi: 10.3390/foods13010028.
Harpiosquillid mantis shrimp () (HMS) without and with beheading pretreated with pulsed electric field (PEF) (15 kV/cm, 800 pulses, 5 min) were soaked in chitooligosaccharide (COS) solution at varying concentrations (0, 1 and 2%, /) for 20 min and stored for 3 days in iced water. Changes in the trypsin activity, color, texture, protein pattern, TCA soluble peptide content, histological images, protein secondary structure and microbial load were monitored during the storage. The beheaded HMS pretreated with PEF and soaked in 2% COS solution showed the maximum efficacy in inhibiting trypsin activity and proteolysis, thus retaining muscle proteins, especially myosin heavy chain, actin and troponin T as well as shear force up to day 3. Pronounced muscle destruction in the whole HMS was displayed by a decreased mean grey index and fiber gapping. Such changes were lowered by the beheading and PEF/2% COS treatment (2% COS-BH). Nevertheless, no marked change in the secondary structure including α-helix, β-sheets, β-turns and random coil were observed among any of the samples. The microbiological analysis revealed that the total viable count (TVC) was below 6 log CFU/g till day 2 in all samples. Nonetheless, the 2% COS-BH sample had the lowest psychrophilic bacterial count and count at day 3, compared to the others. Thus, the combination of the prior PEF and 2% COS treatment of beheaded HMS could effectively inhibit proteases, retard the microbial growth and maintain the quality of HMS stored in iced water.
对经脉冲电场（PEF）（15 kV/cm，800 脉冲，5 分钟）预处理的无头和有头哈氏螳螂虾（Harpiosquillid mantis shrimp）（HMS），分别在不同浓度（0%、1%和 2%，w/v）的壳寡糖（COS）溶液中浸泡 20 分钟，并在冰水中储存 3 天。在储存期间监测胰蛋白酶活性、颜色、质地、蛋白质图谱、三氯乙酸可溶性肽含量、组织学图像、蛋白质二级结构和微生物负荷的变化。经 PEF 预处理并浸泡在 2% COS 溶液中的有头 HMS 在抑制胰蛋白酶活性和蛋白水解方面显示出最大功效，从而在第 3 天之前保留肌肉蛋白质，尤其是肌球蛋白重链、肌动蛋白和肌钙蛋白 T 以及剪切力。整个 HMS 中明显的肌肉破坏表现为平均灰度指数降低和纤维间隙增大。斩首和 PEF/2% COS 处理（2% COS - BH）可减轻此类变化。然而，在任何样品中均未观察到包括α - 螺旋、β - 折叠、β - 转角和无规卷曲在内的二级结构有明显变化。微生物分析表明，所有样品在第 2 天之前总活菌数（TVC）均低于 6 log CFU/g。尽管如此，与其他样品相比，2% COS - BH 样品在第 3 天的嗜冷菌数和[此处原文缺失部分内容]数最低。因此，对有头 HMS 先进行 PEF 处理再用 2% COS 处理的组合可有效抑制蛋白酶、延缓微生物生长并保持储存在冰水中的 HMS 的品质。
