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转录因子 WRKY75 维持生长素稳态以促进番茄对丁香假单胞菌的防御。

Transcription factor WRKY75 maintains auxin homeostasis to promote tomato defense against Pseudomonas syringae.

机构信息

State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai' an, Shandong 271018, China.

School of Landscape Architecture, Beijing Forestry University, No. 35, Qinghua East Road, Haidian District, Beijing 100083, China.

出版信息

Plant Physiol. 2024 May 31;195(2):1053-1068. doi: 10.1093/plphys/kiae025.

Abstract

The hemibiotrophic bacterial pathogen Pseudomonas syringae infects a range of plant species and causes enormous economic losses. Auxin and WRKY transcription factors play crucial roles in plant responses to P. syringae, but their functional relationship in plant immunity remains unclear. Here, we characterized tomato (Solanum lycopersicum) SlWRKY75, which promotes defenses against P. syringae pv. tomato (Pst) DC3000 by regulating plant auxin homeostasis. Overexpressing SlWRKY75 resulted in low free indole-3-acetic acid (IAA) levels, leading to attenuated auxin signaling, decreased expansin transcript levels, upregulated expression of PATHOGENESIS-RELATED GENES (PRs) and NONEXPRESSOR OF PATHOGENESIS-RELATED GENE 1 (NPR1), and enhanced tomato defenses against Pst DC3000. RNA interference-mediated repression of SlWRKY75 increased tomato susceptibility to Pst DC3000. Yeast one-hybrid, electrophoretic mobility shift assays, and luciferase activity assays suggested that SlWRKY75 directly activates the expression of GRETCHEN HAGEN 3.3 (SlGH3.3), which encodes an IAA-amido synthetase. SlGH3.3 enhanced tomato defense against Pst DC3000 by converting free IAA to the aspartic acid (Asp)-conjugated form IAA-Asp. In addition, SlWRKY75 interacted with a tomato valine-glutamine (VQ) motif-containing protein 16 (SlVQ16) in vivo and in vitro. SlVQ16 enhanced SlWRKY75-mediated transcriptional activation of SlGH3.3 and promoted tomato defense responses to Pst DC3000. Our findings illuminate a mechanism in which the SlVQ16-SlWRKY75 complex participates in tomato pathogen defense by positively regulating SlGH3.3-mediated auxin homeostasis.

摘要

丁香假单胞菌是一种兼性生物营养型细菌病原体,可感染多种植物物种,并造成巨大的经济损失。生长素和 WRKY 转录因子在植物对丁香假单胞菌的反应中起着至关重要的作用,但它们在植物免疫中的功能关系尚不清楚。在这里,我们对番茄(Solanum lycopersicum)SlWRKY75 进行了特征描述,该基因通过调节植物生长素稳态促进了对丁香假单胞菌 pv.番茄(Pst)DC3000 的防御。过表达 SlWRKY75 导致游离吲哚-3-乙酸(IAA)水平降低,从而减弱了生长素信号,下调了扩展蛋白转录水平,上调了病程相关基因(PRs)和非表达病程相关基因 1(NPR1)的表达,并增强了番茄对 Pst DC3000 的防御。SlWRKY75 的 RNA 干扰抑制增加了番茄对 Pst DC3000 的易感性。酵母单杂交、电泳迁移率变动分析和荧光素酶活性分析表明,SlWRKY75 可直接激活 GRETCHEN HAGEN 3.3(SlGH3.3)的表达,SlGH3.3 编码一个 IAA-酰胺合成酶。SlGH3.3 通过将游离 IAA 转化为天冬氨酸(Asp)缀合形式 IAA-Asp 来增强番茄对 Pst DC3000 的防御。此外,SlWRKY75 在体内和体外与番茄缬氨酸-谷氨酰胺(VQ)基序包含蛋白 16(SlVQ16)相互作用。SlVQ16 增强了 SlWRKY75 介导的 SlGH3.3 的转录激活,并促进了番茄对 Pst DC3000 的防御反应。我们的研究结果阐明了 SlVQ16-SlWRKY75 复合物通过正向调节 SlGH3.3 介导的生长素稳态参与番茄病原体防御的机制。

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