开发用于预防亚洲海鲈鱼苗和鱼种养殖池塘中链球菌病和柱状病的浸泡式和口服二价纳米疫苗。

Development of Immersion and Oral Bivalent Nanovaccines for Streptococcosis and Columnaris Disease Prevention in Fry and Fingerling Asian Seabass () Nursery Farms.

作者信息

Meachasompop Pakapon, Bunnoy Anurak, Keaswejjareansuk Wisawat, Dechbumroong Piroonrat, Namdee Katawut, Srisapoome Prapansak

机构信息

Laboratory of Aquatic Animal Health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, 50 Paholayothin Rd., Ladyao, Chatuchak, Bangkok 10900, Thailand.

Center of Excellence in Aquatic Animal Health Management, Faculty of Fisheries, Kasetsart University, 50 Paholayothin Rd., Ladyao, Chatuchak, Bangkok 10900, Thailand.

出版信息

Vaccines (Basel). 2023 Dec 22;12(1):17. doi: 10.3390/vaccines12010017.

In the present study, chitosan-based bivalent nanovaccines of and were administered by immersion vaccination at 30 and 40 days after hatching (DAH), and the third vaccination was orally administered by feeding at 50 DAH. ELISA revealed that the levels of total IgM and specific IgM to and were significantly elevated in all vaccinated groups at 10, 20, and 30 days after vaccination (DAV). A qRT-PCR analysis of immune-related genes revealed significantly higher expression in the vaccinated groups compared to the control group, as revealed by 44-100-fold changes in the vaccinated groups compared to the control ( < 0.001) at every tested time point after vaccination. All vaccinated groups expressed , , and at significantly higher levels than the control group at 10 and/or 20 DAV ( < 0.05). In the challenge tests, the survival of vaccinated groups ranged from 62.15 ± 2.11 to 75.70 ± 3.36%, which significantly differed from that of the control group (44.44 ± 1.92%). Similarly, all vaccinated groups showed higher survival rates of 68.89 ± 3.85 to 77.78 ± 5.09% during challenge than the control groups (50.00 ± 3.33%) ( < 0.05).

在本研究中，基于壳聚糖的二价纳米疫苗于孵化后30天和40天通过浸浴接种进行给药，第三次接种于孵化后50天通过投喂口服给药。酶联免疫吸附测定（ELISA）显示，在接种疫苗后10天、20天和30天，所有接种组中针对[具体物质1]和[具体物质2]的总IgM和特异性IgM水平均显著升高。免疫相关基因的定量逆转录聚合酶链反应（qRT-PCR）分析显示，与对照组相比，接种组中[基因名称]的表达显著更高，接种组与对照组相比在接种后每个测试时间点的变化倍数为44 - 100倍（P < 0.001）。在接种疫苗后10天和/或20天，所有接种组中[其他基因名称1]、[其他基因名称2]和[其他基因名称3]的表达水平均显著高于对照组（P < 0.05）。在[具体病原体1]攻毒试验中，接种组的存活率在62.15 ± 2.11%至75.70 ± 3.36%之间，与对照组（44.44 ± 1.92%）有显著差异。同样，在[具体病原体2]攻毒期间，所有接种组的存活率为68.89 ± 3.85%至77.78 ± 5.09%，高于对照组（50.00 ± 3.33%）（P < 0.05）。