Kaňuková Šarlota, Ondreičková Katarína, Mihálik Daniel, Kraic Ján
Department of Applied Biology and Genetics, Research Institute of Plant Production, National Agricultural and Food Centre, Bratislavska cesta 122, 92168 Piestany, Slovakia.
Department of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Namestie J. Herdu 2, 91701 Trnava, Slovakia.
Life (Basel). 2023 Dec 31;14(1):72. doi: 10.3390/life14010072.
L. is cultivated for its edible seeds and for the production of alkaloids. A serious problem in seed trade and processing is the intentional mixing of excellent food-quality seeds with non-food-grade-quality seeds. Tracking the correct or illegitimate handling of seeds requires an efficient method for discrimination and individualization of poppy varieties. As in human and animal forensics, DNA variable regions containing short tandem repeats (STRs) located either in non-coding DNA or in gene sequences (EST-STRs) are preferred markers for discrimination between genotypes. Primers designed for 10 poppy EST-STR loci not analyzed so far were tested for their discriminatory ability on a set of 23 related L. genotypes. Thirty-three EST-STR alleles were identified together. Their polymorphic information content (PIC) values were in the range of 0.175-0.649. The PI value varied in the range of 0.140-0.669, and the cumulative PI was 1.2 × 10. PI values varied between 0.436 and 0.820 and the cumulative value was lower (5.0 × 10). All analyzed genotypes were distinguished mutually, each with its own unique EST-STR profile. These newly developed EST-STR markers more effectively discriminated L. genotypes, even those genotypes whose DNA profiles were previously identical.
丽春花因其可食用种子和生物碱生产而被种植。种子贸易和加工中的一个严重问题是优质食用种子与非食品级种子的故意混合。追踪种子的正确或非法处理需要一种有效的方法来区分和鉴别罂粟品种。与人类和动物法医鉴定一样，位于非编码DNA或基因序列（EST-STRs）中的包含短串联重复序列（STRs）的DNA可变区域是区分基因型的首选标记。针对10个迄今未分析的罂粟EST-STR位点设计的引物，在一组23个相关的丽春花基因型上测试了它们的鉴别能力。共鉴定出33个EST-STR等位基因。它们的多态信息含量（PIC）值在0.175 - 0.649范围内。PI值在0.140 - 0.669范围内变化，累积PI为1.2×10。PI值在0.436和0.820之间变化，累积值较低（5.0×10）。所有分析的基因型相互区分，每个都有其独特的EST-STR图谱。这些新开发的EST-STR标记更有效地鉴别了丽春花基因型，即使是那些以前DNA图谱相同的基因型。
