Camenzind Marcela, Koller Teresa, Armbruster Cygni, Jung Esther, Brunner Susanne, Herren Gerhard, Keller Beat
Department of Plant and Microbial Biology, University of Zurich, Zollikerstrasse 107, 8008 Zurich, Switzerland.
Agroscope, Reckenholzstrasse 191, 8046 Zurich, Switzerland.
Mol Breed. 2024 Jan 22;44(2):8. doi: 10.1007/s11032-024-01451-2. eCollection 2024 Feb.
Breeding for resistant crops is a sustainable way to control disease and relies on the introduction of novel resistance genes. Here, we tested three strategies on how to use transgenes from wheat to achieve durable resistance against fungal pathogens in the field. First, we tested the highly effective, overexpressed single transgene in the background of spring wheat cultivar Bobwhite in a long-term field trial over many years. Together with previous results, this revealed that transgenic wheat line Pm3e#2 conferred complete powdery mildew resistance during a total of nine field seasons without a negative impact on yield. Furthermore, overexpressed provided resistance to powdery mildew isolates from our worldwide collection when crossed into the elite wheat cultivar Fiorina. Second, we pyramided the four overexpressed transgenes , , , and in the background of cultivar Bobwhite and showed that the pyramided line Pm3a,b,d,f was completely resistant to powdery mildew in five field seasons. Third, we performed field trials with three barley lines expressing adult plant resistance gene from wheat during three field seasons. Line GLP8 expressed under control of the pathogen-inducible promoter and provided partial barley powdery mildew and leaf rust resistance in the field with small, negative effects on yield components which might need compensatory breeding. Overall, our study demonstrates and discusses three successful strategies for achieving fungal disease resistance of wheat and barley in the field using transgenes from wheat. These strategies might confer long-term resistance if applied in a sustainable way.
The online version contains supplementary material available at 10.1007/s11032-024-01451-2.
培育抗病作物是控制病害的可持续方法，且依赖于引入新的抗性基因。在此，我们测试了三种利用小麦转基因实现田间对真菌病原体持久抗性的策略。首先，我们在春小麦品种“博白”的背景下，通过多年的长期田间试验测试了高效、过表达的单个转基因。结合先前的结果，这表明转基因小麦品系Pm3e#2在总共九个田间季节中均表现出对白粉病完全抗性，且对产量无负面影响。此外，当导入优良小麦品种“菲奥里纳”时，过表达的 对白粉病菌株的全球收集物具有抗性。其次，我们在“博白”品种背景下将四个过表达转基因 、 、 和 进行聚合，结果表明聚合品系Pm3a,b,d,f在五个田间季节对白粉病完全抗性。第三，我们在三个田间季节对三个表达来自小麦的成株抗性基因 的大麦品系进行了田间试验。品系GLP8在病原体诱导型 启动子的控制下表达 ，在田间对大麦白粉病和叶锈病提供部分抗性，对产量构成因素有较小的负面影响，可能需要进行补偿性育种。总体而言，我们的研究展示并讨论了三种利用小麦转基因在田间实现小麦和大麦真菌病害抗性的成功策略。如果以可持续方式应用，这些策略可能赋予长期抗性。
在线版本包含可在10.1007/s11032-024-01451-2获取的补充材料。
