Zhang Zhibo, Ju Mingxiu, Tang Zhongming, He Zhen, Hua Shengni
Department of Radiation Oncology, Zhuhai People's Hospital, Zhuhai Hospital Affiliated with Jinan University, Zhuhai, China.
Front Immunol. 2024 Jan 11;14:1289548. doi: 10.3389/fimmu.2023.1289548. eCollection 2023.
Hepatocellular carcinoma (HCC) is the most common type of liver cancer, accounting for ~90% of the total cases. DnaJ heat shock protein family member C8 (DNAJC8), belonging to the heat shock protein 40 (HSP40) family, is known to regulate cancer biology function. However, the role of DNAJC8 on HCC development remains unknown.
The Cancer Genome Atlas, GTEx, cBioPortal, and Human Protein Atlas were used to analyze the expression and clinical significance of DNAJC8 in HCC. Two HCC cell lines, MHCC-97H and Huh-7, were utilized to determine the biological function of DNAJC8.
DNAJC8 expression was upregulated in HCC tissues and correlated with poor clinical prognosis. It was closely related to spliceosome, nucleocytoplasmic transport, and cell cycle and might be involved in the formation of tumor immunosuppressive microenvironment. Knockdown of DNAJC8 severely inhibited HCC cell proliferation and induced apoptosis.
Our study demonstrate that functions as an oncogene in HCC and hence may be used as a potential therapeutic target and prognostic marker for HCC.
肝细胞癌（HCC）是最常见的肝癌类型，约占所有病例的90%。DnaJ热休克蛋白家族成员C8（DNAJC8）属于热休克蛋白40（HSP40）家族，已知其可调节癌症生物学功能。然而，DNAJC8在HCC发生发展中的作用尚不清楚。
利用癌症基因组图谱（The Cancer Genome Atlas）、基因型-组织表达数据库（GTEx）、cBioPortal和人类蛋白质图谱（Human Protein Atlas）分析DNAJC8在HCC中的表达及临床意义。使用两种HCC细胞系MHCC-97H和Huh-7来确定DNAJC8的生物学功能。
DNAJC8在HCC组织中表达上调，且与不良临床预后相关。它与剪接体、核质转运和细胞周期密切相关，可能参与肿瘤免疫抑制微环境的形成。敲低DNAJC8可严重抑制HCC细胞增殖并诱导细胞凋亡。
我们的研究表明，DNAJC8在HCC中作为癌基因发挥作用，因此可能用作HCC的潜在治疗靶点和预后标志物。
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