Wang Jing, Wan Yanran, Zhang Ying, Yuan Jiangjiang, Zheng Xiaobin, Cao Hongyi, Qian Kanghua, Feng Jiuming, Tang Yingxi, Chen Sirui, Zhang Youjun, Zhou Xuguo, Liang Pei, Wu Qingjun
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Department of Entomology, China Agricultural University, Beijing 100193, China.
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
J Hazard Mater. 2024 Mar 15;466:133575. doi: 10.1016/j.jhazmat.2024.133575. Epub 2024 Jan 20.
Uridine diphosphate glucosyltransferases (UGTs) play crucial roles in the insect detoxification system and are associated with pesticide resistance. Our previous transcriptomic analysis of spinosad-susceptible (Ivf03) and resistant (NIL-R) Frankliniella occidentalis revealed numerous upregulated UGT genes in the NIL-R strain, suggesting their potential contribution to spinosad resistance. To investigate this hypothesis, here we conducted UGT activity assays and spinosad induction experiments, employing RNA interference (RNAi) techniques for gene function validation. We found significantly elevated UGT activity in the NIL-R strain compared to Ivf03, with 5-nitrouracil showing a substantial synergistic effect on the resistant strain. Eighteen UGT genes were identified in F. occidentalis, with gene expansion and duplication observed within families UGT466, 467, and 468. Ten out of the eighteen UGTs exhibited higher expression levels in NIL-R, specifically FoUGT466B1, FoUGT468A3, and FoUGT468A4 consistently being upregulated across nymphs, males, and females. RNAi-based functional validation targeting these three UGT genes led to increased susceptibility to spinosad in a life stage-, sex-, and dose-dependent manner. These results indicate that UGTs are indeed involved in spinosad resistance in F. occidentalis, and the effects are dependent on life stage, sex, and dose. Therefore, sustainable control for F. occidentalis resistance should always consider these differential responses.
尿苷二磷酸葡萄糖基转移酶（UGTs）在昆虫解毒系统中发挥着关键作用，并与抗药性相关。我们之前对西花蓟马敏感品系（Ivf03）和抗性品系（NIL-R）进行的转录组分析显示，NIL-R品系中有大量UGT基因上调，表明它们对多杀菌素抗性具有潜在贡献。为了验证这一假设，我们进行了UGT活性测定和多杀菌素诱导实验，并采用RNA干扰（RNAi）技术进行基因功能验证。我们发现，与Ivf03相比，NIL-R品系中的UGT活性显著升高，5-硝基尿嘧啶对抗性品系表现出显著的协同作用。在西花蓟马中鉴定出18个UGT基因，在UGT466、467和468家族中观察到基因扩增和重复。18个UGT中有10个在NIL-R中表达水平较高，特别是FoUGT466B1、FoUGT468A3和FoUGT468A4在若虫、雄虫和雌虫中均持续上调。针对这三个UGT基因的基于RNAi的功能验证导致对多杀菌素的敏感性以生活阶段、性别和剂量依赖性方式增加。这些结果表明，UGTs确实参与了西花蓟马对多杀菌素的抗性，且其影响取决于生活阶段、性别和剂量。因此，对西花蓟马抗性的可持续控制应始终考虑这些差异反应。
