中国浙江省与茶树枯萎病相关的镰刀菌属的鉴定及致病性

Identification and pathogenicity of Fusarium spp. associated with tea wilt in Zhejiang Province, China.

作者信息

Tang Zhaoyang, Zhu Jing, Song Qiujin, Daly Paul, Kong Liya, He Luqian, Li Agen, Lou Jun, Wang Zhanqi, Zhang Liqin, Min Lijing

机构信息

Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, Huzhou, 313000, China.

Zhejiang Zhongyi Testing Institute Co., Ltd., Huzhou, 313000, China.

出版信息

BMC Microbiol. 2024 Jan 27;24(1):38. doi: 10.1186/s12866-023-03174-4.

DOI:10.1186/s12866-023-03174-4

PMID:38281024

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10821546/

Abstract

BACKGROUND

Tea is one of the most widely consumed beverages in the world, with significant economic and cultural value. However, tea production faces many challenges due to various biotic and abiotic stresses, among which fungal diseases are particularly devastating.

RESULTS

To understand the identity and pathogenicity of isolates recovered from tea plants with symptoms of wilt, phylogenetic analyses and pathogenicity assays were conducted. Isolates were characterized to the species level by sequencing the ITS, tef-1α, tub2 and rpb2 sequences and morphology. Four Fusarium species were identified: Fusarium fujikuroi, Fusarium solani, Fusarium oxysporum, and Fusarium concentricum. The pathogenicity of the Fusarium isolates was evaluated on 1-year-old tea plants, whereby F. fujikuroi OS3 and OS4 strains were found to be the most virulent on tea.

CONCLUSIONS

To the best of our knowledge, this is the first report of tea rot caused by F. fujikuroi in the world. This provides the foundation for the identification and control of wilt disease in tea plants.

摘要

背景

茶是世界上消费最为广泛的饮品之一，具有重要的经济和文化价值。然而，由于各种生物和非生物胁迫，茶叶生产面临诸多挑战，其中真菌病害尤其具有毁灭性。

结果

为了解从出现枯萎症状的茶树中分离出的菌株的特性和致病性，进行了系统发育分析和致病性测定。通过对ITS、tef-1α、tub2和rpb2序列进行测序以及形态学分析，将分离菌株鉴定到种水平。鉴定出四种镰刀菌：藤仓镰刀菌、茄病镰刀菌、尖孢镰刀菌和轮枝镰刀菌。在一年生茶树上评估了镰刀菌分离株的致病性，结果发现藤仓镰刀菌OS3和OS4菌株对茶树的毒性最强。