Olson D M, Shimada K, Etches R J
Biol Reprod. 1986 Dec;35(5):1140-6. doi: 10.1095/biolreprod35.5.1140.
An increase in the plasma concentrations of prostaglandins (PGs) is associated with uterine contractile activity and with oviposition in the hen. In order to assess the contribution of potential sources of prostaglandins to the increase in prostaglandin levels observed at oviposition, prostaglandins E2, F2 alpha, and 13,14-dihydro-15-keto PGF2 alpha (PGFM, the stable but biologically less active metabolite of PGF2 alpha) were measured in plasma from the brachial vein, ovarian follicular vein and uterine vein, and in tissues from ovarian follicles and the uterus 12 h before and at midsequence oviposition or a terminal oviposition. These two ovipositions differ in that a midsequence oviposition is followed within 0.25-1.0 h by the next ovulation of the sequence, whereas the terminal oviposition is followed by an ovulation 14 h later. The concentration of PGFM in plasma from the brachial vein increased at midsequence oviposition, while the levels of PGE2 were unchanged. Prostaglandin E2, F2 alpha, and FM levels were each similar in the plasma from the brachial and uterine veins at the time of midsequence oviposition. In plasma from the largest preovulatory follicle, the concentration of PGF2 alpha and PGFM increased 19- and 7-fold, respectively, from 12 h before midsequence oviposition to midsequence oviposition, although no changes were observed in the concentrations of PGE2 during this interval. The levels of PGF2 alpha increased in the tissues of the two largest preovulatory follicles and the two most recently ruptured follicles during the 12-h period before a midsequence oviposition, while there was no change or a decrease in PGE2 levels in these tissues during the same interval. In contrast, the concentration of PGF2 alpha did not increase during the 12-h period preceding the terminal oviposition of the sequence in plasma from the brachial, uterine, or follicular veins.(ABSTRACT TRUNCATED AT 250 WORDS)
前列腺素（PGs）血浆浓度的升高与母鸡的子宫收缩活动及排卵有关。为了评估前列腺素潜在来源对排卵时观察到的前列腺素水平升高的贡献，在序列排卵中期或末期排卵前12小时及排卵中期，测量了来自臂静脉、卵巢卵泡静脉和子宫静脉的血浆以及卵巢卵泡和子宫组织中的前列腺素E2、F2α和13,14 - 二氢 - 15 - 酮PGF2α（PGFM，PGF2α的稳定但生物活性较低的代谢产物）。这两种排卵的不同之处在于，序列排卵中期之后0.25 - 1.0小时内会接着发生该序列的下一次排卵，而末期排卵之后14小时才会排卵。序列排卵中期时，臂静脉血浆中PGFM的浓度升高，而PGE2的水平未变。序列排卵中期时，臂静脉和子宫静脉血浆中的前列腺素E2、F2α和FM水平各自相似。在最大的排卵前卵泡的血浆中，从序列排卵中期前12小时到序列排卵中期，PGF2α和PGFM的浓度分别增加了19倍和7倍，尽管在此期间PGE2的浓度没有变化。在序列排卵中期前的12小时内，两个最大的排卵前卵泡和两个最近破裂的卵泡组织中PGF2α的水平升高，而在同一期间这些组织中PGE2的水平没有变化或下降。相比之下，在序列末期排卵前的12小时内，臂静脉、子宫静脉或卵泡静脉血浆中PGF2α的浓度没有增加。（摘要截断于250字）
