Wang Shuo, Han Hui, Lei Xiaoqing, Ma Jianxiang, Tao Ze, Ren Yamei
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China.
Int J Biol Macromol. 2024 Mar;261(Pt 1):129777. doi: 10.1016/j.ijbiomac.2024.129777. Epub 2024 Jan 28.
In this study, the cellulose nanofibers (CNFs) derived from spaghetti squash peel (SSP) were prepared using a novel approach involving deep eutectic solvent (DES) pretreatment coupled with ultrasonication. Molecular dynamics (MD) simulations revealed that the number of hydrogen bonds influences the viscosity and density of DES systems, and experimental viscosity (η) confirmed consistency with the computed viscosity (η) trends. After DES pretreatment and ultrasonication, the cellulose content of ChCl/oxalic acid (ChCl/OA) CNF (35.63%) and ChCl/formic acid (ChCl/FA) (32.46%) is higher than ChCl/Urea CNF (28.27%). The widths of ChCl/OA CNF, ChCl/FA CNF, and ChCl/Urea CNF were 19.83, 11.34, and 18.27 nm, respectively, showing a network-like fiber distribution. Compared with SSP (29.76%) and non-ultrasonic samples, the crystallinity index of ChCl/OA CNF, ChCl/FA CNF, and ChCl/Urea CNF was improved by ultrasonication. The thermal decomposition residue of ChCl/OA CNF (25.54%), ChCl/FA CNF (18.54%), and ChCl/Urea CNF (23.62%) was lower than that of SSP (29.57%). These results demonstrate that CNFs can be prepared from SSP via DES pretreatment combined with ultrasonication. The lowest viscosity observed in the formic acid DES group (η of 18 mPa·s), the ChCl/FA CNF exhibits excellent stability (Zeta potential of -37.6 mV), which can provide a promising prospect for utilization in biomass by-products and applications in the materials field.
在本研究中，采用了一种新方法制备源自意大利面南瓜皮（SSP）的纤维素纳米纤维（CNF），该方法涉及深共熔溶剂（DES）预处理与超声处理相结合。分子动力学（MD）模拟表明，氢键数量会影响DES体系的粘度和密度，实验粘度（η）证实与计算得到的粘度（η）趋势一致。经过DES预处理和超声处理后，氯化胆碱/草酸（ChCl/OA）CNF（35.63%）和氯化胆碱/甲酸（ChCl/FA）（32.46%）的纤维素含量高于氯化胆碱/尿素CNF（28.27%）。ChCl/OA CNF、ChCl/FA CNF和ChCl/尿素CNF的宽度分别为19.83、11.34和18.27nm，呈现出网络状纤维分布。与SSP（29.76%）和非超声处理样品相比，超声处理提高了ChCl/OA CNF、ChCl/FA CNF和ChCl/尿素CNF的结晶度指数。ChCl/OA CNF（25.54%）、ChCl/FA CNF（18.54%）和ChCl/尿素CNF（23.62%）的热分解残渣低于SSP（29.57%）。这些结果表明，可以通过DES预处理结合超声处理从SSP制备CNF。在甲酸DES组中观察到最低粘度（η为18mPa·s），ChCl/FA CNF表现出优异的稳定性（ζ电位为-37.6mV），这为生物质副产品的利用和材料领域的应用提供了广阔前景。
