Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Science, Jilin University, Changchun, Jilin 130062, China.
CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, China.
Zool Res. 2024 Mar 18;45(2):253-262. doi: 10.24272/j.issn.2095-8137.2023.201.
Animal models are extensively used in all aspects of biomedical research, with substantial contributions to our understanding of diseases, the development of pharmaceuticals, and the exploration of gene functions. The field of genome modification in rabbits has progressed slowly. However, recent advancements, particularly in CRISPR/Cas9-related technologies, have catalyzed the successful development of various genome-edited rabbit models to mimic diverse diseases, including cardiovascular disorders, immunodeficiencies, aging-related ailments, neurological diseases, and ophthalmic pathologies. These models hold great promise in advancing biomedical research due to their closer physiological and biochemical resemblance to humans compared to mice. This review aims to summarize the novel gene-editing approaches currently available for rabbits and present the applications and prospects of such models in biomedicine, underscoring their impact and future potential in translational medicine.
动物模型在生物医学研究的各个方面都得到了广泛的应用，为我们理解疾病、开发药物和探索基因功能做出了巨大贡献。兔基因组修饰领域的发展一直较为缓慢。然而，最近的进展，特别是在 CRISPR/Cas9 相关技术方面，推动了各种基因组编辑兔模型的成功开发，以模拟包括心血管疾病、免疫缺陷、与年龄相关的疾病、神经疾病和眼科疾病在内的多种疾病。与小鼠相比，这些模型在生理和生化上更接近人类，因此在推进生物医学研究方面具有很大的潜力。本综述旨在总结目前用于兔的新型基因编辑方法，并介绍这些模型在生物医学中的应用和前景，强调它们在转化医学中的影响和未来潜力。
