Chen Letian, Zhang Dongdong, Chen Yujuan, Zhu Huilan, Liu Zhipeng, Yu Zhiping, Xie Junping
Department of Pulmonary and Critical Care Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China.
Department of General Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China.
Oncol Lett. 2024 Jan 11;27(3):96. doi: 10.3892/ol.2024.14229. eCollection 2024 Mar.
Origin recognition complexes (ORCs) are vital in the control of DNA replication and the progression of the cell cycle, however the precise function and mechanism of ORC6 in non-small cell lung cancer (NSCLC) is still not well understood. The present study used bioinformatics methods to assess the predictive significance of ORC6 expression in NSCLC. Moreover, the expression of ORC6 was further evaluated using reverse transcription-quantitative PCR and western blotting, and its functional significance in lung cancer was assessed via knockdown experiments using small interfering RNA. A significant association was demonstrated between the expression of ORC6 and the clinical features of NSCLC. In particular, elevated levels of ORC6 were significantly strongly correlated with an unfavorable prognosis. Multivariate analysis demonstrated that increased ORC6 expression independently contributed to the risk of overall survival (HR 1.304; P=0.015) in individuals diagnosed with NSCLC. Analysis of Kaplan-Meier plots demonstrated that ORC6 expression served as a valuable indicator for diagnosing and predicting the prognosis of NSCLC. Moreover, studies demonstrated that modified ORC6 expression had a significant impact on the proliferation, migration and metastasis of NSCLC cells. NSCLC cell lines (H1299 and mH1650) exhibited markedly higher ORC6 expression than normal lung cell lines. The results of the present study indicated a strong association between the expression of ORC6 and the clinicopathological characteristics of NSCLC, which suggested its potential as a reliable biomarker for predicting NSCLC. Furthermore, ORC6 may have important therapeutic implications in the management of NSCLC.
起始识别复合物（ORCs）在DNA复制的控制和细胞周期进程中至关重要，然而ORC6在非小细胞肺癌（NSCLC）中的精确功能和机制仍未完全明确。本研究采用生物信息学方法评估ORC6表达在NSCLC中的预测意义。此外，通过逆转录定量PCR和蛋白质印迹进一步评估ORC6的表达，并使用小干扰RNA通过敲低实验评估其在肺癌中的功能意义。结果表明，ORC6的表达与NSCLC的临床特征之间存在显著关联。特别是，ORC6水平升高与不良预后显著相关。多变量分析表明，ORC6表达增加独立地导致了NSCLC患者总生存风险（HR 1.304；P = 0.015）。Kaplan-Meier曲线分析表明，ORC6表达可作为诊断和预测NSCLC预后的有价值指标。此外，研究表明，改变ORC6表达对NSCLC细胞的增殖、迁移和转移有显著影响。NSCLC细胞系（H1299和mH1650）的ORC6表达明显高于正常肺细胞系。本研究结果表明，ORC6表达与NSCLC的临床病理特征密切相关，提示其有可能作为预测NSCLC的可靠生物标志物。此外，ORC6可能在NSCLC的治疗中具有重要意义。
