Tanjila Nargis, Islam Shaikhul, Akhter Md Shamim, Hossain Md Monzur, Alam Mohammad Shahidul, Begum Ferdousi
Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi, 6205 Bangladesh.
Plant Pathology Division, Bangladesh Wheat and Maize Research Institute (BWMRI), Nashipur, Dinajpur, 5200 Bangladesh.
3 Biotech. 2024 Feb;14(2):58. doi: 10.1007/s13205-023-03890-8. Epub 2024 Jan 30.
The development of the foot rot disease caused by the fungus is one of the primary variables endangering betel vine production in Bangladesh. Consequently, with the ultimate objective of finding efficient preventive and control strategies for this infamous phytopathogen, the current study was undertaken for comprehensive population structure analysis, exploration of physiological features and incidence patterns of pathogenic isolates. We discovered 22 isolates from nine northern districts of Bangladesh Mohanpur (51.90%), Bagmara (54.09%), and Durgapur (49.45%) upazilas in the Rajshahi district had the more severe occurrences of foot rot disease, while Chapainawabganj (18.89%) had the least number of cases. The isolates differed substantially in terms of morphology and growth rate. By employing the UPGMA algorithm to analyze the combined morphological data from 22 isolates, these isolates were divided into six different groups with a 62% similarity level. Somatic incompatibility was also found in some isolates. The RAPD-4 primer confirmed 100% polymorphism among these isolates, and these genetic variations were further validated by molecular analysis. The results of the morphological and molecular analysis revealed that there was significant variation among the isolates. Finally, a comprehensive characterization of would allow for a suitable management strategy for betel vine's deadly foot rot disease.
The online version contains supplementary material available at 10.1007/s13205-023-03890-8.
由该真菌引起的脚腐病的发展是危及孟加拉国槟榔藤生产的主要变量之一。因此，为了找到针对这种臭名昭著的植物病原体的有效预防和控制策略，本研究旨在进行全面的种群结构分析、探索致病分离株的生理特征和发病模式。我们从孟加拉国北部九个地区发现了22株分离株，拉杰沙希地区的莫汉布尔（51.90%）、巴格马拉（54.09%）和杜尔加布尔（49.45%）乡的脚腐病发病情况更为严重，而查帕伊瑙瓦布甘杰（18.89%）的病例数最少。这些分离株在形态和生长速率方面有很大差异。通过使用UPGMA算法分析22株分离株的综合形态数据，这些分离株被分为六个不同的组，相似性水平为62%。在一些分离株中还发现了体细胞不亲和性。RAPD - 4引物证实这些分离株之间存在100%的多态性，并且这些遗传变异通过分子分析得到了进一步验证。形态学和分子分析结果表明，这些分离株之间存在显著差异。最后，对[未提及的相关事物]进行全面表征将有助于制定针对槟榔藤致命脚腐病的合适管理策略。
在线版本包含可在10.1007/s13205 - 023 - 03890 - 8获取的补充材料。
