评估人诱导多能干细胞系用于临床的胰腺β细胞分化效率。
Evaluation of Pancreatic β-cell Differentiation Efficiency of Human iPSC Lines for Clinical Use.
机构信息
Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1, Komaba, Meguro-ku, Tokyo, 153-8902, Japan.
出版信息
Curr Stem Cell Res Ther. 2024;19(11):1449-1460. doi: 10.2174/011574888X267226231126185532.
BACKGROUND
Transplantation of pancreatic β-cells generated from human induced pluripotent stem cells (hiPSCs) has great potential as a root treatment for type 1 diabetes. However, their current level of efficiency to differentiate into β-cells is still not at par for clinical use. Previous research has shown that differentiation efficiency varies among human embryonic stem cells and mouse-induced pluripotent stem cell lines. Therefore, selecting a suitable cell line for efficient induction into desired tissues and organs is crucial.
METHODS
In this study, we have evaluated the efficiency of 15 hiPSC lines available for clinical use to differentiate into pancreatic β-cells.
RESULTS
Our investigation has revealed induction efficiency to differ among the hiPSC lines, even when derived from the same donor. Among the hiPSC lines tested, the 16A01 cell line exhibited the highest expression and low expression, suggesting that this cell line is suitable for differentiation into β-cells.
CONCLUSION
Our study has demonstrated the importance of selecting a suitable hiPSC line for effective differentiation into β-cells.
背景
从人诱导多能干细胞（hiPSCs）中产生的胰腺β细胞移植具有作为 1 型糖尿病根本治疗的巨大潜力。然而，其目前分化为β细胞的效率仍不能达到临床应用的要求。以前的研究表明，人胚胎干细胞和小鼠诱导多能干细胞系之间的分化效率存在差异。因此，选择合适的细胞系进行有效的诱导分化为所需的组织和器官至关重要。
方法
在这项研究中，我们评估了 15 种可用于临床的 hiPSC 系分化为胰腺β细胞的效率。
结果
我们的研究表明，即使来自同一供体，hiPSC 系之间的诱导效率也存在差异。在测试的 hiPSC 系中，16A01 细胞系表现出最高的 表达和低的 表达，这表明该细胞系适合分化为β细胞。
结论
我们的研究表明，选择合适的 hiPSC 系对于有效地分化为β细胞非常重要。
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