Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environment Sciences, Ministry of Ecology and Environment, Guangdong 510655, China; Chongqing Key Laboratory of Water Environment Evolution and Pollution Control in the Three Gorges Reservoir Area, Chongqing Three Gorges University, Chongqing 404000, China.
Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environment Sciences, Ministry of Ecology and Environment, Guangdong 510655, China; Guangdong-Hong Kong-Macao Joint Laboratory for Contaminants Exposure and Health, Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou 510006, China.
Ecotoxicol Environ Saf. 2023 Jan 1;249:114450. doi: 10.1016/j.ecoenv.2022.114450. Epub 2022 Dec 19.
Biochar-immobilized bacteria have been widely used to remove organic pollutants; however, the enhanced effect of biochar-immobilized bacteria on tetrabromobisphenol A (TBBPA) removal has not been fully investigated and the removal mechanism remains unclear. In this study, a bacterial strain with high TBBPA degradation ability, Burkholderia cepacian Y17, was isolated from an e-waste disassembly area, immobilized with biochar, and used for the removal of TBBPA. Comparisons were performed of the factors affecting the immobilization and TBBPA removal efficiency, including the biochar preparation temperature, immobilization temperature, and pH. The highest 7-day TBBPA removal efficiency by immobilized bacteria was observed with the most suitable biochar preparation temperature (BC500) and an immobilization pH and temperature of 7 and 35 °C, respectively. The TBBPA removal efficiency reached 59.37%, which was increased by 30.23% and 15.88% compared to that of free and inactivated immobilized Y17, respectively. The suitable biochar preparation temperature BC500, immobilization temperature of 35 °C, and neutral pH of 7 increased the bacterial population and extracellular polymer concentration, which facilitated bacterial immobilization on biochar and promoted TBBPA removal. In this case, the high immobilized bacteria concentration (4.62 × 10 cfu∙g) and protein and polysaccharide contents (28.43 and 16.16 mg·g) contributed to the removal of TBBPA by facilitating TBBPA degradation. The main TBBPA degradation processes by BC500-immobilized Y17 involved debromination, β-scission, demethylation, O-methylation, hydroxylation, and hydroxyl oxidation. This study proposes a method for preparing immobilized bacteria for TBBPA removal and enriches the microbial degradation technology for TBBPA.
生物炭固定化细菌已被广泛用于去除有机污染物；然而，生物炭固定化细菌对四溴双酚 A（TBBPA）去除的增强效果尚未得到充分研究，去除机制仍不清楚。本研究从电子废物拆解区分离出一株具有高 TBBPA 降解能力的细菌，即洋葱伯克霍尔德菌 Y17，并用生物炭对其进行固定化，用于去除 TBBPA。比较了影响固定化和 TBBPA 去除效率的因素，包括生物炭制备温度、固定化温度和 pH 值。固定化细菌的 7 天 TBBPA 去除效率最高，最适生物炭制备温度（BC500）、固定化 pH 值和温度分别为 7 和 35°C。TBBPA 去除效率达到 59.37%，分别比游离和失活固定化 Y17 提高了 30.23%和 15.88%。合适的生物炭制备温度 BC500、35°C 的固定化温度和中性 pH 值 7 增加了细菌数量和胞外聚合物浓度，有利于细菌在生物炭上的固定化，并促进了 TBBPA 的去除。在这种情况下，高固定化细菌浓度（4.62×10 cfu·g）和蛋白质及多糖含量（28.43 和 16.16 mg·g）有助于通过促进 TBBPA 降解来去除 TBBPA。BC500 固定化 Y17 去除 TBBPA 的主要过程包括脱溴、β 断裂、脱甲基、O-甲基化、羟化和羟基氧化。本研究提出了一种用于去除 TBBPA 的固定化细菌的制备方法，丰富了 TBBPA 的微生物降解技术。
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