Baniya Anil, Subkrasae Chanakan, Ardpairin Jiranun, Anesko Kyle, Vitta Apichat, Dillman Adler R
Department of Nematology, University of California, Riverside, California 92521.
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.
J Parasitol. 2024 Feb 1;110(1):22-39. doi: 10.1645/23-60.
A new species of entomopathogenic nematode, Steinernema adamsi n. sp., was recovered from the soil of a longan tree (Dimocarpus sp.) in Mueang Lamphun District, Thailand, using baiting techniques. Upon analysis of the nematode's morphological traits, we found it to be a new species of Steinernema and a member of the Longicaudatum clade. Molecular analyses of the ITS rDNA and D2D3 of 28S rDNA sequences further confirmed that S. adamsi n. sp. is a new species of the Longicaudatum clade, which is closely related to Steinernema guangdongense and Steinernema longicaudam. Using morphometric analysis, the infective juveniles measure between 774.69 and 956.96 μm, males have a size range of 905.44 to 1,281.98 μm, and females are within the range of 1,628.21 to 2,803.64 μm. We also identified the symbiotic bacteria associated with the nematode based on 16S sequences as Xenorhabdus spp. closely related toXenorhabdus griffiniae. Furthermore, we have successfully assessed a cryopreservation method for the long-term preservation of S. adamsi n. sp. Successful cryopreservation of this new species will allow for the longer preservation of its traits and will be valuable for its future use. The discovery of this new species has significant implications for the development of effective biological control agents in Thailand, and our work contributes to our understanding of the diversity and evolution of entomopathogenic nematodes.
利用诱饵技术，从泰国南奔府直辖县一棵龙眼树（Dimocarpus sp.）的土壤中分离出一种新的昆虫病原线虫——亚当斯斯氏线虫（Steinernema adamsi n. sp.）。通过对线虫形态特征的分析，我们发现它是斯氏线虫属的一个新物种，属于长尾斯氏线虫进化枝。对ITS rDNA和28S rDNA序列的D2D3进行分子分析，进一步证实亚当斯斯氏线虫是长尾斯氏线虫进化枝的一个新物种，与广东斯氏线虫和长尾斯氏线虫密切相关。通过形态测量分析，感染期幼虫体长在774.69至956.96μm之间，雄虫体长范围为905.44至1281.98μm，雌虫体长在1628.21至2803.64μm之间。我们还根据16S序列鉴定了与该线虫共生的细菌为嗜线虫致病杆菌属（Xenorhabdus spp.），与格里菲斯嗜线虫致病杆菌（Xenorhabdus griffiniae）密切相关。此外，我们成功评估了一种用于长期保存亚当斯斯氏线虫的冷冻保存方法。成功冷冻保存这个新物种将使其特性得以更长期保存，对其未来应用具有重要价值。这一新物种的发现对泰国开发有效的生物防治剂具有重要意义，我们的工作有助于增进我们对昆虫病原线虫多样性和进化的理解。
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